Longitudinal immunosequencing in healthy people reveals persistent T cell receptors rich in highly public receptors
| Autor: | Harlan Robins, Nathaniel D. Chu, Anna Sherwood, Michael E. Birnbaum, Eric J. Alm, Ryan O. Emerson, Haixin Sarah Bi |
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| Rok vydání: | 2019 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
0301 basic medicine Time Factors Naive T cell T-Lymphocytes T cell Immunology Memory T cell Adaptive Immunity Biology Lymphocyte Activation 03 medical and health sciences 0302 clinical medicine Immune system Species Specificity Antigen T-Lymphocyte Subsets medicine Humans Repertoire sequencing Public receptors Clonal Selection Antigen-Mediated Healthy controls Cells Cultured Repertoire T-cell receptor High-Throughput Nucleotide Sequencing Cell Differentiation Biodiversity Acquired immune system Persistent receptors Healthy Volunteers Immunosequencing 030104 developmental biology medicine.anatomical_structure Genes T-Cell Receptor beta T cell receptor lcsh:RC581-607 Immunologic Memory Research Article 030215 immunology |
| Zdroj: | BMC Immunology, Vol 20, Iss 1, Pp 1-12 (2019) BMC Immunology |
| ISSN: | 1471-2172 |
| Popis: | Background The adaptive immune system maintains a diversity of T cells capable of recognizing a broad array of antigens. Each T cell’s specificity for antigens is determined by its T cell receptors (TCRs), which together across all T cells form a repertoire of millions of unique receptors in each individual. Although many studies have examined how TCR repertoires change in response to disease or drugs, few have explored the temporal dynamics of the TCR repertoire in healthy individuals. Results Here we report immunosequencing of TCR β chains (TCRβ) from the blood of three healthy individuals at eight time points over one year. TCRβ repertoires of all peripheral-blood T cells and sorted memory T cells clustered clearly by individual, systematically demonstrating that TCRβ repertoires are specific to individuals across time. This individuality was absent from TCRβs from naive T cells, suggesting that the differences resulted from an individual’s antigen exposure history, not genetic background. Many characteristics of the TCRβ repertoire (e.g., diversity, clonality) were stable across time, although we found evidence of T cell expansion dynamics even within healthy individuals. We further identified a subset of “persistent” TCRβs present across all time points. These receptors were rich in clonal and highly public receptors and may play a key role in immune system maintenance. Conclusions Our results highlight the importance of longitudinal sampling of the immune system, providing a much-needed baseline for TCRβ dynamics in healthy individuals. Such a baseline will improve interpretation of changes in the TCRβ repertoire during disease or treatment. Electronic supplementary material The online version of this article (10.1186/s12865-019-0300-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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