Protective effects and potential underlying mechanisms of sodium copper chlorophyllin against ethanol-induced gastric ulcer in mice
| Autor: | Huawei Lv, Kemin Wei, Pei-Gang Liu, Liang Weiqing, Yan Lin, Hongjian Zhang, Pu Jinbao |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_treatment
Biophysics Pharmacology medicine.disease_cause Nitric Oxide Biochemistry Proinflammatory cytokine 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Western blot Malondialdehyde medicine Animals Stomach Ulcer Omeprazole 030304 developmental biology Peroxidase bcl-2-Associated X Protein 0303 health sciences Mice Inbred BALB C medicine.diagnostic_test biology Chlorophyllides Ethanol Chemistry Interleukin-6 Tumor Necrosis Factor-alpha Transcription Factor RelA General Medicine Cytokine Proto-Oncogene Proteins c-bcl-2 Apoptosis 030220 oncology & carcinogenesis Myeloperoxidase biology.protein Female Oxidative stress medicine.drug |
| Zdroj: | Acta biochimica et biophysica Sinica. 51(9) |
| ISSN: | 1745-7270 |
| Popis: | In study, we aimed to determine the mechanisms underlying the gastroprotective effects of sodium copper chlorophyllin (SCC) against ethanol-induced gastric ulcer injury in mice. First, the gastroprotective effects of SCC against gastric ulcer induced by ethanol were assessed. Then, biochemical, histopathological, immunohistochemistry assays, and western blot analysis were conducted to determine the possible mechanisms of action underlying the effects of SCC. Compared to the effects of omeprazole (OME) in a confirmed mouse model of ethanol-induced gastric ulcer injury, treatment with various doses of SCC resulted in up-regulation of Bcl-2 and down-regulation of the pro-apoptotic protein Bax. Significant decreases in the levels of the malondialdehyde (MDA), myeloperoxidase (MPO), and NO in the gastric tissues were observed. Furthermore, inflammatory cytokine analysis revealed that SCC treatment inhibited the expressions of TNF-α and IL-6, greatly reduced the phosphorylation level of IκB, and repressed the nuclear translocation of NF-κB p65, which demonstrated that SCC inhibited the activation of the NF-κB pathway. The present findings suggest that the protective effects of SCC may be beneficial as a potential preventive and therapeutic agent for gastric ulcer through the NF-κB pathway. Taken together, SCC administration significantly decreased the levels of MPO, NO, and MDA in gastric tissue and exerted a powerful anti-inflammatory activity as demonstrated by reduction in the secretions of proinflammatory mediators such as IL-6 and TNF-α in the serum of mice exposed to ethanol. |
| Databáze: | OpenAIRE |
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