Alterations in Auxin Homeostasis Suppress Defects in Cell Wall Function
| Autor: | Blaire Steinwand, Joseph J. Kieber, Joanna K. Polko, Shou-Ling Xu, Mike Westafer, Stephanie M. Doctor |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
Mutant Arabidopsis lcsh:Medicine Plant Science 01 natural sciences Biochemistry Lignin Plant Roots Cell Wall Molecular Cell Biology Homeostasis lcsh:Science chemistry.chemical_classification Plant Growth and Development 0303 health sciences Multidisciplinary biology Plant Biochemistry food and beverages Plants Cell biology Crosstalk (biology) Benzamides Signal transduction Cellular Structures and Organelles Plant Cell Walls Pyruvate Decarboxylase Research Article Plant Cell Biology Arabidopsis Thaliana Brassica Research and Analysis Methods Cell wall 03 medical and health sciences Cell Walls Model Organisms Auxin Plant and Algal Models Botany Cellulose 030304 developmental biology Cell Proliferation Auxin homeostasis Indoleacetic Acids Cell growth Arabidopsis Proteins lcsh:R fungi Organisms Biology and Life Sciences Cell Biology biology.organism_classification chemistry Mutation lcsh:Q Protein Kinases 010606 plant biology & botany Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 5, p e98193 (2014) |
| DOI: | 10.17615/eyrk-3t16 |
| Popis: | The plant cell wall is a highly dynamic structure that changes in response to both environmental and developmental cues. It plays important roles throughout plant growth and development in determining the orientation and extent of cell expansion, providing structural support and acting as a barrier to pathogens. Despite the importance of the cell wall, the signaling pathways regulating its function are not well understood. Two partially redundant leucine-rich-repeat receptor-like kinases (LRR-RLKs), FEI1 and FEI2, regulate cell wall function in Arabidopsis thaliana roots; disruption of the FEIs results in short, swollen roots as a result of decreased cellulose synthesis. We screened for suppressors of this swollen root phenotype and identified two mutations in the putative mitochondrial pyruvate dehydrogenase E1α homolog, IAA-Alanine Resistant 4 (IAR4). Mutations in IAR4 were shown previously to disrupt auxin homeostasis and lead to reduced auxin function. We show that mutations in IAR4 suppress a subset of the fei1 fei2 phenotypes. Consistent with the hypothesis that the suppression of fei1 fei2 by iar4 is the result of reduced auxin function, disruption of the WEI8 and TAR2 genes, which decreases auxin biosynthesis, also suppresses fei1 fei2. In addition, iar4 suppresses the root swelling and accumulation of ectopic lignin phenotypes of other cell wall mutants, including procuste and cobra. Further, iar4 mutants display decreased sensitivity to the cellulose biosynthesis inhibitor isoxaben. These results establish a role for IAR4 in the regulation of cell wall function and provide evidence of crosstalk between the cell wall and auxin during cell expansion in the root. |
| Databáze: | OpenAIRE |
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