DNA damage interactions on both nanometer and micrometer scale determine overall cellular damage

Autor: Thomas Friedrich, Judith Reindl, Stefanie Girst, Christoph Greubel, Christian Siebenwirth, Michael Scholz, K. Ilicic, Matthias Sammer, Thomas E. Schmid, Dietrich W. M. Walsh, Günther Dollinger, Benjamin Schwarz
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
Scientific reports 8(1), 16063 (2018). doi:10.1038/s41598-018-34323-9
Sci. Rep. 8:16063 (2018)
Scientific Reports
ISSN: 2045-2322
Popis: DNA double strand breaks (DSB) play a pivotal role for cellular damage, which is a hazard encountered in toxicology and radiation protection, but also exploited e.g. in eradicating tumors in radiation therapy. It is still debated whether and in how far clustering of such DNA lesions leads to an enhanced severity of induced damage. Here we investigate - using focused spots of ionizing radiation as damaging agent - the spatial extension of DNA lesion patterns causing cell inactivation. We find that clustering of DNA damage on both the nm and µm scale leads to enhanced inactivation compared to more homogeneous lesion distributions. A biophysical model interprets these observations in terms of enhanced DSB production and DSB interaction, respectively. We decompose the overall effects quantitatively into contributions from these lesion formation processes, concluding that both processes coexist and need to be considered for determining the resulting damage on the cellular level.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje