On-line two-dimensional capillary electrophoresis with mass spectrometric detection using a fully electric isolated mechanical valve
| Autor: | Felix J. Kohl, Christian Neusüß, Cristina Montealegre |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Bioanalysis
Materials science Clinical Biochemistry Analytical chemistry 02 engineering and technology Electrolyte Mass spectrometry 01 natural sciences Biochemistry Capillary electrophoresis–mass spectrometry Mass Spectrometry Analytical Chemistry Switching time Capillary electrophoresis Dimension (vector space) Ionization Electrophoresis Gel Two-Dimensional Chromatography 010401 analytical chemistry Electrophoresis Capillary Serum Albumin Bovine Equipment Design 021001 nanoscience & nanotechnology Peptide Fragments 0104 chemical sciences Feasibility Studies 0210 nano-technology |
| Zdroj: | ELECTROPHORESIS. 37:954-958 |
| ISSN: | 0173-0835 |
| DOI: | 10.1002/elps.201500579 |
| Popis: | CE is becoming more and more important in many fields of bioanalytical chemistry. Besides optical detection, hyphenation to ESI-MS detection is increasingly applied for sensitive identification purposes. Unfortunately, many CE techniques and methods established in research and industry are not compatible to ESI-MS since essential components of the background electrolyte interfere in ES ionization. In order to identify unknown peaks in established CE methods, here, a heart-cut 2D-CE separation system is introduced using a fully isolated mechanical valve with an internal loop of only 20 nL. In this system, the sample is separated using potentially any non-ESI compatible method in the first separation dimension. Subsequently, the portion of interest is cut by the internal sample loop of the valve and reintroduced to the second dimension where the interfering compounds are removed, followed by ESI-MS detection. When comparing the separation efficiency of the system with the valve to a system using a continuous capillary only a slight increase in peak width is observed. Ultraviolet/visible detection is integrated in the first dimension for switching time determination, enabling reproducible cutting of peaks of interest. The feasibility of the system is successfully demonstrated by a 2D analysis of a BSA tryptic digest sample using a nonvolatile (phosphate based) background electrolyte in the first dimension. |
| Databáze: | OpenAIRE |
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