Examination of lipid profiles in abdominal fascial healing using MALDI-TOF to identify potential therapeutic targets

Autor: Nicole D. Bouvy, Marion J.J. Gijbels, Audrey C. H. M. Jongen, Hong Liu, Ron M. A. Heeren, Jianhua Cao, Benjamin Balluff, Jarno Melenhorst
Přispěvatelé: Surgery, RS: NUTRIM - R2 - Liver and digestive health, Imaging Mass Spectrometry (IMS), RS: M4I - Imaging Mass Spectrometry (IMS), Pathologie, Moleculaire Genetica, RS: Carim - B07 The vulnerable plaque: makers and markers, RS: GROW - R2 - Basic and Translational Cancer Biology, MUMC+: MA Heelkunde (9), Medical Biochemistry, ACS - Atherosclerosis & ischemic syndromes
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Pathology
medicine.medical_treatment
Clinical Biochemistry
H&E stain
MULTICENTER
Abdominal wall
Laparotomy
PHOSPHATIDYLCHOLINE
Medicine
PE
Phosphatidylethanolamine
Fascia
skin and connective tissue diseases
GROWTH-FACTORS
Incisional hernia
Spectroscopy
PI
Phosphatidylinositol
Lipids
Medical Laboratory Technology
CerPE
Ceramide phosphorylethanolamine
medicine.anatomical_structure
LPC
Lysophosphatidylcholine
PHOSPHATIDYLETHANOLAMINE METABOLISM
lipids (amino acids
peptides
and proteins)
HEALTH
PA
Phosphatidic acid
medicine.medical_specialty
PC
Phosphatidylcholine
AA
Arachidonic acid
BIOLOGY
Wound healing
Abdominal fascia
Microbiology
Special issue on Lipidomics
GM3
Monosialodihexosylganglioside
Mass spectrometry imaging
Medical technology
R855-855.5
Fibroblast
SM
Sphingomyelin
business.industry
CYTOKINES
medicine.disease
MMPE
Monomethyl-phosphatidylethanolamine
CLOSURE
sense organs
CL
Cardiolipin
MEMBRANE
business
LPA
Lysophosphatidic acid
Zdroj: Journal of Mass Spectrometry and Advances in the Clinical Lab, Vol 20, Iss, Pp 35-41 (2021)
Journal of Mass Spectrometry and Advances in the Clinical Lab, 20, 35-41. Elsevier
Journal of Mass Spectrometry and Advances in the Clinical Lab
Journal of Mass Spectrometry and Advances in the Clinical Lab, 20, 35-41. Elsevier BV
ISSN: 2667-145X
Popis: Highlights • Lipids change overtime in normal fascial healing in the early post-surgery period. • Specific lipid species are correlated with the changes of inflammation cells and fibroblasts. • Lipid species in the present study are considered as predictive markers for the formation of incisional hernia.
Background Failure of fascial healing in the abdominal wall can result in incisional hernia, which is one of the most common complications after laparotomy. Understanding the molecular healing process of abdominal fascia may provide lipid markers of incisional hernia or therapeutic targets that allow prevention or treatment of incisional hernias. Purpose This study aims to investigate temporal and in situ changes of lipids during the normal healing process of abdominal fascia in the first postoperative week. Methods Open hemicolectomy was performed in a total of 35 Wistar rats. The midline fascia was closed identically for all rats using a single continuous suturing technique. These animals were sacrificed with equal numbers (n = 5) at each of 7-time points (6, 12, 24, 48, 72, 120, and 168 h. The local and temporal changes of lipids were examined with mass spectrometry imaging and correlated to histologically scored changes during healing using hematoxylin and eosin staining. Results Two phosphatidylcholine lipid species (PC O-38:5 and PC 38:4) and one phosphatidylethanolamine lipid (PE O-16:1_20:4) were found to significantly correlate with temporal changes of inflammation. A phosphatidylcholine (PC 32:0) and a monosialodihexosylganglioside (GM3 34:1;2) were found to correlate with fibroblast cell growth. Conclusion Glycerophospholipids and gangliosides are strongly involved in the normal healing process of abdominal fascia and their locally fluctuating concentrations are considered as potential lipid markers and therapeutic targets of fascial healing.
Databáze: OpenAIRE
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