Characterization of indigenous rhizobia from Caatinga
| Autor: | Gustavo Ribeiro Xavier, Norma Gouvêa Rumjanek, Fernanda Cíntia Pires e Teixeira, Wardsson Lustrino Borges |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Mimosa tenuiflora
Root nodule PCR-ARDRA Cratylia mollis lcsh:QR1-502 Ribosomal RNA Biology biology.organism_classification Calliandra depauperata Microbiology Bradyrhizobium lcsh:Microbiology Rhizobia diversity Industrial Microbiology Restriction enzyme Botany Nitrogen fixation Ribosomal DNA Research Paper |
| Zdroj: | Brazilian Journal of Microbiology v.41 n.1 2010 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Volume: 41, Issue: 1, Pages: 201-208, Published: MAR 2010 Brazilian Journal of Microbiology, Vol 41, Iss 1, Pp 201-208 (2010) |
| Popis: | The aim of this study was to characterize rhizobial isolates from Cratylia mollis Mart. ex Benth, Calliandra depauperata Benth. and Mimosa tenuiflora (Willd.) Poir. by means of rhizobial colonies morphology and restriction analysis of the 16S ribosomal gene (16S rDNA-ARDRA). Nodules were collected in the field and from plants cultivated in a greenhouse experiment using Caatinga soil samples. Sixty seven isolates were described by morphological analysis. Forty seven representative isolates were used for ARDRA analysis using seven restriction enzymes. We observed high diversity of both slow and fast-growing rhizobia that formed three morpho-physiological clusters. A few fast-growing isolates formed a group of strains of the Bradyrhizobium type; however, most of them diverged from the B. japonicum and B. elkanii species. Cratylia mollis nodule isolates were the most diverse, while all Mimosa tenuiflora isolates displayed fast growth with no pH change and were clustered into groups bearing 100% similarity, according to ARDRA results. |
| Databáze: | OpenAIRE |
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