Effects of quercetin on the release of endothelin, prostacyclin and tissue plasminogen activator from human endothelial cells in culture
| Autor: | Xueying Zhao, Zhenlun Gu, Chun-Su Yuan, Anoja S. Attele |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Endothelium Prostacyclin 6-Ketoprostaglandin F1 alpha Biology chemistry.chemical_compound Thrombin Internal medicine Drug Discovery medicine Humans heterocyclic compounds Platelet activation Cells Cultured Pharmacology Endothelin-1 T-plasminogen activator Platelet Activation Endothelin 1 Endothelial stem cell medicine.anatomical_structure Endocrinology chemistry Tissue Plasminogen Activator Quercetin Endothelium Vascular medicine.drug |
| Zdroj: | Journal of Ethnopharmacology. 67:279-285 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/s0378-8741(99)00055-0 |
| Popis: | Quercetin and related flavonoids are naturally occurring polyphenolic compounds with multiple pharmacological activities. Using cultured human umbilical vein endothelial cells, we investigated the effects of quercetin on endothelin (ET-1) and tissue plasminogen activator (t-PA) release induced by thrombin. We observed that when endothelial cells pretreated with 5 or 50 microM of quercetin were incubated for 4 and 24 h with thrombin, ET-1 concentration-dependently decreased (n = 6, P < 0.01, at 4 h IC50 = 1.54 microM, at 24 h IC50 = 2.78 microM). Under the same experimental conditions, quercetin significantly increased t-PA (n = 6, P < 0.01, at 4 h EC50 = 0.71 microM and at 24 hrs EC50 = 0.74 microM). In the same preparation, we evaluated prostacyclin (PGI2) release, induced by thrombin activated platelets, as determined by a 6-Keto-PGF1alpha radioimmunoassay. Following the treatment of cultured endothelial cells with activated platelets, the concentration of 6-Keto-PGF1alpha was significantly increased (P < 0.01). Quercetin (1, 5, and 20 microM) inhibited PGI2, in a concentration-dependent manner (n = 6, P < 0.05). Our data indicate that quercetin modulates the release of ET-1, t-PA, and PGI2 from vascular endothelial cells. |
| Databáze: | OpenAIRE |
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