Long-term profile of serological biomarkers, hepatic inflammation, and fibrosis in a mouse model of non-alcoholic fatty liver disease
| Autor: | Jeong Hun Kang, Riki Toita |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Liver Cirrhosis
Male 0301 basic medicine medicine.medical_specialty Normal diet Blood lipids Toxicology Hepatitis Pathogenesis Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Liver Function Tests Non-alcoholic Fatty Liver Disease Fibrosis Internal medicine Animals Medicine Triglycerides Triglyceride business.industry Body Weight Cholesterol HDL Fatty liver General Medicine Lipid Metabolism medicine.disease Diet Mice Inbred C57BL Cholesterol 030104 developmental biology Endocrinology Liver chemistry Steatohepatitis business Hepatic fibrosis Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | Toxicology Letters. 332:1-6 |
| ISSN: | 0378-4274 |
| Popis: | Non-alcoholic fatty liver disease (NAFLD) can be typically classified into two subgroups: non-alcoholic fatty liver and non-alcoholic steatohepatitis. Mouse models of NAFLD are useful tools for understanding the pathogenesis and progression of NAFLD and for developing drugs for its treatment. Here, we investigated the time-dependent changes in serum lipids and biochemical markers of hepatic function, hepatic inflammation, and fibrosis in mice fed a normal diet (ND) or a NAFLD diet (choline deficient, L-amino acid-defined, high-fat diet; CDAHFD) for 12 weeks. CDAHFD-fed mice showed significantly reduced serum levels of total cholesterol, triglyceride, and high-density lipoprotein cholesterol throughout the treatment period compared with ND-fed mice. The changes in aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, and total bilirubin showed an inverse U-shaped curve in the CDAHFD-fed mice. The serum alkaline phosphatase levels decreased in both ND- and CDAHFD-fed mice in a time-dependent manner. Furthermore, CDAHFD-fed mice showed a significant increase in the number of inflammatory foci and hepatic fibrosis at 6-12 weeks, although inflammatory foci and hepatic fibrogenesis were observable at relatively early stages as well (1-4 weeks). In conclusion, the long-term profile of serological biomarkers, hepatic inflammation, and fibrosis in CDAHFD-fed mice identified in this study may provide a better understanding of NAFLD pathogenesis. |
| Databáze: | OpenAIRE |
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