The assembly of developing motor neurons depends on an interplay between spontaneous activity, type II cadherins and gap junctions
| Autor: | Ana Uzquiano, Andrew J. Lowe, Sarah Guthrie, Karli Montague, Stephen R. Price, Athene Knüfer, Marc Astick |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Central Nervous System Research Report Central nervous system Amino Acid Motifs Chick Embryo Biology 03 medical and health sciences Mice 0302 clinical medicine medicine Cell Adhesion Image Processing Computer-Assisted Animals Cell adhesion Molecular Biology Cell Nucleus Motor Neurons Cadherin Gap junction Gap Junctions Anatomy Cadherins 030104 developmental biology medicine.anatomical_structure NIH 3T3 Cells Calcium Brainstem Neuron Neuroscience Nucleus 030217 neurology & neurosurgery Developmental Biology Brain Stem |
| Zdroj: | Development (Cambridge, England). 144(5) |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | A core structural and functional motif of the vertebrate central nervous system is discrete clusters of neurons or ‘nuclei’. Yet the developmental mechanisms underlying this fundamental mode of organisation are largely unknown. We have previously shown that the assembly of motor neurons into nuclei depends on cadherin-mediated adhesion. Here, we demonstrate that the emergence of mature topography among motor nuclei involves a novel interplay between spontaneous activity, cadherin expression and gap junction communication. We report that nuclei display spontaneous calcium transients, and that changes in the activity patterns coincide with the course of nucleogenesis. We also find that these activity patterns are disrupted by manipulating cadherin or gap junction expression. Furthermore, inhibition of activity disrupts nucleogenesis, suggesting that activity feeds back to maintain integrity among motor neurons within a nucleus. Our study suggests that a network of interactions between cadherins, gap junctions and spontaneous activity governs neuron assembly, presaging circuit formation. |
| Databáze: | OpenAIRE |
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