Circulating Tumor Cell Count Correlates with Colorectal Neoplasm Progression and Is a Prognostic Marker for Distant Metastasis in Non-Metastatic Patients
| Autor: | Tsung-Fu Hung, Jinn-Shiun Chen, Hsin-Yuan Hung, Pao-Shiu Hsieh, Hung-Jen Shao, Reiping Tang, Si-Hong Lu, Geng-Ping Lin, Chien-Yuh Yeh, Jeng-Fu You, Ying-Chih Chang, Jen-chia Wu, Jr-Ming Lai, Wen-Sy Tsai, Sum-Fu Chiang, Yen-Chi Chiu |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology Adult Male medicine.medical_specialty Pathology Colorectal cancer Microfluidics Circulating Tumor Cell Count Cell Count Article 03 medical and health sciences 0302 clinical medicine Circulating tumor cell Internal medicine medicine Non metastatic Humans Neoplasm Metastasis Aged Aged 80 and over Nonspecific binding Multidisciplinary business.industry Distant metastasis Cancer Middle Aged medicine.disease Neoplastic Cells Circulating Prognosis digestive system diseases 030104 developmental biology Colorectal neoplasm 030220 oncology & carcinogenesis Female business Colorectal Neoplasms |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Enumeration of circulating tumor cells (CTCs) has been proven as a prognostic marker for metastatic colorectal cancer (m-CRC) patients. However, the currently available techniques for capturing and enumerating CTCs lack of required sensitivity to be applicable as a prognostic marker for non-metastatic patients as CTCs are even more rare. We have developed a microfluidic device utilizing antibody-conjugated non-fouling coating to eliminate nonspecific binding and to promote the multivalent binding of target cells. We then established the correlation of CTC counts and neoplasm progression through applying this platform to capture and enumerate CTCs in 2 mL of peripheral blood from healthy (n = 27), benign (n = 21), non-metastatic (n = 95), and m-CRC (n = 15) patients. The results showed that the CTC counts progressed from 0, 1, 5, to 36. Importantly, after 2-year follow-up on the non-metastatic CRC patients, we found that those who had ≥5 CTCs were 8 times more likely to develop distant metastasis within one year after curable surgery than those who had |
| Databáze: | OpenAIRE |
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