QTL mapping for traits associated with stress neuroendocrine reactivity in rats

Autor: Bastien Llamas, Hubert Vaudry, V. Contesse, Pierre Mormède, Véronique Guyonnet-Dupérat, Marie-Pierre Moisan
Přispěvatelé: Neurogénétique et Stress (NS), Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)
Rok vydání: 2005
Předmět:
Male
Angiotensin receptor
Oligonucleotides
Pituitary-Adrenal System
Plasma renin activity
Receptors
G-Protein-Coupled
Renin-Angiotensin System
chemistry.chemical_compound
0302 clinical medicine
Corticosterone
Renin
Receptor
Aldosterone
HORMONE STEROIDE
ComputingMilieux_MISCELLANEOUS
Apelin Receptors
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
Chromosome Mapping
Radioimmunoassay
Organ Size
HPA
Female
Hypothalamo-Hypophyseal System
medicine.medical_specialty
Genotype
Quantitative Trait Loci
HYPOTHALAMIC-PITUITARY-ADRENAL AXIS
Thymus Gland
Quantitative trait locus
Biology
03 medical and health sciences
Stress
Physiological
Internal medicine
Renin–angiotensin system
Genetics
medicine
Animals
Crosses
Genetic
030304 developmental biology
Analysis of Variance
[SDV.GEN]Life Sciences [q-bio]/Genetics
Rats
Inbred Strains
Sequence Analysis
DNA
Rats
Endocrinology
chemistry
RAT
Lod Score
030217 neurology & neurosurgery
Zdroj: Mammalian Genome
Mammalian Genome, Springer Verlag, 2005, 16 (7), pp.505-515. ⟨10.1007/s00335-005-0022-2⟩
ISSN: 1432-1777
0938-8990
DOI: 10.1007/s00335-005-0022-2
Popis: In the present study we searched for quantitative trait loci (QTLs) that affect neuroendocrine stress responses in a 20-min restraint stress paradigm using Brown-Norway (BN) and Wistar-Kyoto-Hyperactive (WKHA) rats. These strains differed in their hypothalamic-pituitary-adrenal axis (plasma ACTH and corticosterone levels, thymus, and adrenal weights) and in their renin-angiotensin-aldosterone system reactivity (plasma renin activity, aldosterone concentration). We performed a whole-genome scan on a F2 progeny derived from a WKHA x BN intercross, which led to the identification of several QTLs linked to plasma renin activity (Sr6, Sr8, Sr11, and Sr12 on chromosomes RNO2, 3, 19, and 8, respectively), plasma aldosterone concentration (Sr7 and Sr9 on RNO2 and 5, respectively), and thymus weight (Sr10, Sr13, and Srl4 on RNO5, 10, and 16, respectively). The type 1b angiotensin II receptor gene (Agtrlb) maps within the confidence intervals of QTLs on RNO2 linked to plasma renin activity (Sr6, highly significant; LOD = 5.0) and to plasma aldosterone level (Sr7, suggestive; LOD = 2.0). In vitro studies of angiotensin II-induced release of aldosterone by adrenal glomerulosa cells revealed a lower receptor potency (log EC50 = -8.16 +/- 0.11 M) and efficiency (Emax = 453.3 +/- 25.9 pg/3 x 10(4) cells/24 h) in BN than in WKHA (log EC50 = -10.66 +/- 0.18 M; Emax = 573.1 +/- 15.3 pg/3 x 10(4) cells/24 h). Moreover, differences in Agtr1b mRNA abundance and sequence reinforce the putative role of the Agtr1b gene in the differential plasma renin stress reactivity between the two rat strains.
Databáze: OpenAIRE
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