RORγt+ cells selectively express redundant cation channels linked to the Golgi apparatus
| Autor: | Gaelle Beriou, Aurélie Lemoine, Melanie Lancien, Aurélie Moreau, Arnaud Nicot, Régis Josien, Jerome Martin, Elise Chiffoleau, Laurence Bouchet-Delbos, Géraldine Bienvenu, Thierry Cens, Maria-Cristina Cuturi, Pierre Charnet, Hans Jörg Fehling, Lucile Drujont, Cédric Louvet, Flora Guillot |
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| Přispěvatelé: | Centre de Recherche en Transplantation et Immunologie (U1064 Inserm - CRTI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Institut de transplantation urologie-néphrologie (ITUN), Université de Nantes (UN)-Centre hospitalier universitaire de Nantes (CHU Nantes), Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1), Institute of Immunology [Ulm], Universitätsklinikum Ulm - University Hospital of Ulm, Faculté de Médecine - Université de Nantes, Laboratoire d'Immunologie [CHU Nantes], Centre hospitalier universitaire de Nantes (CHU Nantes), The IHU-Cesti project is also supported by Nantes Métropole and région Pays de la Loire. Inserm-Région Pays de la Loire fellowship, Fondation Progreffe., ANR-11-LABX-0016,IGO,Immunothérapies Grand Ouest(2011), ANR-10-IBHU-0005,CESTI (TSI-IHU),Centre Européen des Sciences de la Transplantation et de l'Immunothérapie (TSI-IHU)(2010), Centre de recherche en Biologie cellulaire de Montpellier (CRBM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Le Bihan, Sylvie, Laboratoires d'excellence - Immunothérapies Grand Ouest - - IGO2011 - ANR-11-LABX-0016 - LABX - VALID, Instituts Hospitalo-Universitaires B - Centre Européen des Sciences de la Transplantation et de l'Immunothérapie (TSI-IHU) - - CESTI (TSI-IHU)2010 - ANR-10-IBHU-0005 - IBHU - VALID |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Encephalomyelitis Autoimmune Experimental T cell Biology Article 03 medical and health sciences Mice Downregulation and upregulation RAR-related orphan receptor gamma medicine Animals Humans Psoriasis Cells Cultured Orphan receptor [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology Multidisciplinary Experimental autoimmune encephalomyelitis Innate lymphoid cell Membrane Proteins T-Lymphocytes Helper-Inducer Nuclear Receptor Subfamily 1 Group F Member 3 medicine.disease Colitis Cell biology Disease Models Animal 030104 developmental biology medicine.anatomical_structure Intracellular Cation channel activity [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology trans-Golgi Network |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2016, 6 (1), pp.23682. ⟨10.1038/srep23682⟩ Scientific Reports, 2016, 6 (1), pp.23682. ⟨10.1038/srep23682⟩ |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep23682⟩ |
| Popis: | Retinoid-related orphan receptor gamma t (RORγt) is a master transcription factor central to type 17 immunity involving cells such as T helper 17, group 3 innate lymphoid cells or IL-17-producing γδ T cells. Here we show that the intracellular ion channel TMEM176B and its homologue TMEM176A are strongly expressed in these RORγt+ cells. We demonstrate that TMEM176A and B exhibit a similar cation channel activity and mainly colocalise in close proximity to the trans-Golgi network. Strikingly, in the mouse, the loss of Tmem176b is systematically associated with a strong upregulation of Tmem176a. While Tmem176b single-deficiency has no effect on the course of experimental autoimmune encephalomyelitis, T cell or DSS-induced colitis, it significantly reduces imiquimod-induced psoriasis-like skin inflammation. These findings shed light on a potentially novel specific process linked to post-Golgi trafficking for modulating the function of RORγt+ cells and indicate that both homologues should be simultaneously targeted to clearly elucidate the role of this intracellular ion flow. |
| Databáze: | OpenAIRE |
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