Select de novo gene and protein expression during renal epithelial cell culture in rotating wall vessels is shear stress dependent
| Autor: | R. R. Majewski, J. H. Kaysen, Thomas J. Goodwin, W. C. Campbell, Gabriel L. Navar, Fawn C. Lewis, F. O. Goda, Timothy G. Hammond |
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| Rok vydání: | 1999 |
| Předmět: |
Cell type
Kidney Cortex Rotation Physiology Cellular differentiation Response element Biophysics Cell Culture Techniques Heymann Nephritis Antigenic Complex Cell Count Receptors Cell Surface Endosomes Biology Kidney Tubules Proximal Rats Sprague-Dawley Heat shock protein Shear stress Animals Humans Heat-Shock Proteins Regulation of gene expression Membrane Glycoproteins Reverse Transcriptase Polymerase Chain Reaction Superoxide Dismutase Microfilament Proteins Cell Differentiation Epithelial Cells Cell Biology Prostheses and Implants Oligonucleotides Antisense Cell biology Rats Endothelial stem cell Gene Expression Regulation Cell culture Stress Mechanical Carrier Proteins Cell Adhesion Molecules Gravitation |
| Zdroj: | The Journal of membrane biology. 168(1) |
| ISSN: | 0022-2631 |
| Popis: | The rotating wall vessel has gained popularity as a clinical cell culture tool to produce hormonal implants. It is desirable to understand the mechanisms by which the rotating wall vessel induces genetic changes, if we are to prolong the useful life of implants. During rotating wall vessel culture gravity is balanced by equal and opposite hydrodynamic forces including shear stress. The current study provides the first evidence that shear stress response elements, which modulate gene expression in endothelial cells, are also active in epithelial cells. Rotating wall culture of renal cells changes expression of select gene products including the giant glycoprotein scavenger receptors cubulin and megalin, the structural microvillar protein villin, and classic shear stress response genes ICAM, VCAM and MnSOD. Using a putative endothelial cell shear stress response element binding site as a decoy, we demonstrate the role of this sequence in the regulation of selected genes in epithelial cells. However, many of the changes observed in the rotating wall vessel are independent of this response element. It remains to define other genetic response elements modulated during rotating wall vessel culture, including the role of hemodynamics characterized by 3-dimensionality, low shear and turbulence, and cospatial relation of dissimilar cell types. |
| Databáze: | OpenAIRE |
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