31P-NMR study of different hypothyroid states in rat leg muscle
| Autor: | Pierre Kaminsky, Marc Klein, François Brunotte, Jean Marie Escanyé, M. Duc, Paul Walker, Jacques Robert, B. Robin-Lherbier |
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| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Thyroid Hormones Magnetic Resonance Spectroscopy Bioenergetics Physiology Endocrinology Diabetes and Metabolism Intracellular pH Phosphocreatine chemistry.chemical_compound Hypothyroidism Physiology (medical) Internal medicine medicine Pi Animals Glycolysis Muscles Thyroid Body Weight Rats Inbred Strains Metabolism Hydrogen-Ion Concentration Hindlimb Rats Endocrinology medicine.anatomical_structure chemistry Phosphomonoesters |
| Zdroj: | The American journal of physiology. 261(6 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | Using phosphorus nuclear magnetic resonance spectroscopy, this study was undertaken to determine the effects of experimental hypothyroidism on muscle bioenergetics. The peaks of phosphocreatine (PCr), Pi, phosphodiesters (PDE), sugar phosphomonoesters, and ATP were obtained at rest, during a 2-Hz hindleg muscle stimulation, and during a subsequent recovery period from four groups of anesthetized rats as follows: one control and three hypothyroid (HT) groups treated by propylthyouracil during 2, 4, and 6 wk, respectively. Resting spectra showed a significant rise in Pi by 30% and decreased intracellular pH and PCr/Pi in all three HT groups. PDE progressively increased to 200% of its initial value with hypothyroidism duration. Muscle stimulation did not lead to significant differences in PCr depletion. The percentage of PCr recovery is less in HT muscle than in control muscle. An abnormal H+ metabolism is obvious in all three HT groups. These results indicate abnormal bioenergetics in HT muscle and suggest an impairment of mitochondrial metabolism and of the H+ efflux. They also evoke a high sensitivity of cellular energetics to thyroid deficiency. |
| Databáze: | OpenAIRE |
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