Endotoxin stimulates monocyte–endothelial cell interactions in mouse intestinal Peyer's patches and villus mucosa
| Autor: | A. Kawaguchi, Kouji Matsuzaki, Ryota Hokari, Kazurou Itoh, S. Miura, Jyunichi Miyazaki, Yoshikiyo Okada, N Ishii, Yoshikazu Tsuzuki, Shigeaki Nagao |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Lipopolysaccharides
Male P-selectin Immunology Intercellular Adhesion Molecule-1 Vascular Cell Adhesion Molecule-1 Biology Monocytes chemistry.chemical_compound Leukocyte Count Mice Peyer's Patches Intestinal mucosa medicine Cell Adhesion Immunology and Allergy Animals VCAM-1 Intestinal Mucosa Cell adhesion Mice Inbred BALB C Cell adhesion molecule Monocyte Antibodies Monoclonal Endothelial Cells Immunohistochemistry Peyer Patch P-Selectin medicine.anatomical_structure chemistry Animal Studies |
| Popis: | SUMMARY Although monocyte–endothelial cell interactions represent an initial step in controlling the recruitment of monocytes in inflamed tissues, their dynamic processes in microvessels of lymphoid (Peyer's patches) and non-lymphoid (villus) regions in gut-associated lymphoid tissue remain poorly understood. We monitored the migration of fluorescence-labelled monocytes derived from the spleen in intestinal microvessels with or without lipopolysaccharide (LPS) treatment and investigated the role of adhesion molecules, P-selectin, vascular cell adhesion molecule (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1). In control mice, there were few interactions between infused monocytes and the endothelium of intestinal microvessels. The monocyte–endothelial interactions (both rolling and adhesion) were significantly increased in intestinal microvessels of LPS-treated mice compared with those in controls. Anti-P-selectin monoclonal antibody (MoAb) significantly suppressed the LPS-induced increase in monocyte rolling in postcapillary venules of Peyer's patches and submucosal venules. Anti-VCAM-1 MoAbs significantly suppressed the LPS-induced increase in monocyte adhesion to postcapillary venules (PCVs) of Peyer's patches, submucosal venules, and villus capillaries. In contrast, anti-ICAM-1 MoAb significantly suppressed the number of adherent monocytes in PCV of Peyer's patches but not in submucosal venules or villus capillaries. These observations demonstrated that LPS treatment resulted in a significant increase in recruitment of monocytes both in microvessels of lymphoid and non-lymphoid regions and that P-selectin, VCAM-1 and ICAM-1 appeared to play important roles in LPS-induced interactions. |
| Databáze: | OpenAIRE |
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