Collagen-based matrices improve the delivery of transplanted circulating progenitor cells: development and demonstration by ex vivo radionuclide cell labeling and in vivo tracking with positron-emission tomography
| Autor: | Rob S. Beanlands, Marc Lamoureux, Erik J. Suuronen, Robert A. deKemp, Stephanie Thorn, Yan Zhang, Marc Ruel, Jean N. DaSilva |
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| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Biodistribution Cell therapy Rats Sprague-Dawley Tissue engineering In vivo Cell Movement Fluorodeoxyglucose F18 Medicine Animals Humans Radiology Nuclear Medicine and imaging Progenitor cell Tissue Engineering Tissue Scaffolds business.industry Stem Cells Hindlimb Rats Transplantation Positron-Emission Tomography Collagen Stem cell Radiopharmaceuticals Cardiology and Cardiovascular Medicine business Ex vivo Stem Cell Transplantation |
| Zdroj: | Circulation. Cardiovascular imaging. 1(3) |
| ISSN: | 1942-0080 |
| Popis: | Background— Collagen delivery matrices have been reported to improve the results of cell therapy, but knowledge of their mechanisms of action is limited. To evaluate whether a collagen matrix improves early engraftment posttransplantation, 2-[ 18 F]fluoro-2-deoxy- d -glucose ( 18 F-FDG) was used to label transplanted circulating progenitor cells (CPCs) and track them in vivo with positron-emission tomography. Methods and Results— Efficiency of 18 F-FDG cell labeling was CPC-concentration dependent ( r =0.61, P 18 F-FDG-dose dependent. Labeled human CPCs (2�10 6 ) were injected with or without a collagen-based matrix in the ischemic hind limb of rats (n=12 per group) 2 weeks after femoral artery ligation. Imaging of labeled cells, acquired by small animal positron-emission tomography at 150 minutes postinjection, revealed greater CPC retention in the ischemic hind limb and less nonspecific leakage to other tissues (retention ratio, 0.44�0.08) when CPCs were delivered within the matrix, compared with cells injected alone (0.22�0.13, P =0.040) and with 18 F-FDG injected with or without the matrix (0.10�0.05 and 0.11�0.05, respectively, P 18 F-FDG-labeled cells were injected with the collagen matrix, accumulation was significantly increased (by 69.6%, P =0.021) in the target ischemic hind limb muscle and significantly reduced (by 14.8% to 31.4%, P Conclusions— Early posttransplantation, a collagen matrix enhances progenitor cell retention and limits distribution to nonspecific tissues, as measured by the use of 18 F-FDG labeled cells and positron-emission tomography imaging and confirmed by biodistribution and histology. |
| Databáze: | OpenAIRE |
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