Chinese Angelica Polysaccharide (CAP) Alleviates LPS-Induced Inflammation and Apoptosis by Down-Regulating COX-1 in PC12 Cells

Autor: Hai-Tao Zhang, Zheng Wang, Yun-Jie Xie, Deyi Duan, Yang Zhang, Chong Wang
Rok vydání: 2018
Předmět:
Lipopolysaccharides
0301 basic medicine
Angelica sinensis
Physiology
Interleukin-1beta
Cell
Down-Regulation
Apoptosis
Spinal cord injury
Chinese angelica polysaccharide
PC12 Cells
lcsh:Physiology
lcsh:Biochemistry
Phosphatidylinositol 3-Kinases
03 medical and health sciences
0302 clinical medicine
Western blot
Polysaccharides
medicine
Animals
lcsh:QD415-436
Viability assay
Medicine
Chinese Traditional
Cell damage
PI3K/AKT/mTOR pathway
Angelica
bcl-2-Associated X Protein
Inflammation
lcsh:QP1-981
medicine.diagnostic_test
biology
COX-1
Caspase 3
Interleukin-6
Tumor Necrosis Factor-alpha
Chemistry
Transfection
biology.organism_classification
medicine.disease
Rats
Cell biology
030104 developmental biology
medicine.anatomical_structure
Proto-Oncogene Proteins c-bcl-2
030220 oncology & carcinogenesis
Cyclooxygenase 1
Proto-Oncogene Proteins c-akt
Signal Transduction
Zdroj: Cellular Physiology and Biochemistry, Vol 49, Iss 4, Pp 1380-1388 (2018)
ISSN: 1421-9778
1015-8987
DOI: 10.1159/000493415
Popis: Background/Aims: Chinese angelica polysaccharide (CAP) is the main effective ingredient of angelica sinensis and exerts anti-inflammatory and anti-apoptotic effects on many diseases. This study aimed to explore the pharmacological potential of CAP on spinal cord injury (SCI). Methods: PC12 cells were pretreated by CAP and were subjected to LPS. Transfection was performed to alter the expression of COX-1. Cell viability and apoptotic cell rate were measured by CCK-8 and flow cytometry respectively. qRT-PCR and western blot analysis were performed to assess the expression changes of pro-inflammatory cytokines, apoptosis-related factor and core kinases in PI3K/AKT pathway. Results: LPS stimulation induced significant cell damage in PC12 cells as cell viability was repressed, apoptosis was induced and the expression levels of IL-1β, IL-6, IL-8, and TNF-α were increased. CAP pretreatment protected PC12 cells against LPS-induced cell damage. Meanwhile CAP treatment reduced the expression of COX-1 even in LPS-stimulated PC12 cells. More importantly, COX-1 overexpression abolished the protective effects of CAP on LPS-injured PC12 cells. Finally, Western blot analytical results showed that CAP activated PI3K/AKT pathway also in a COX-1-dependent manner. Conclusion: CAP exerted anti-apoptotic and anti-inflammatory effects on LPS-injured PC12 cells via down-regulation of COX-1.
Databáze: OpenAIRE
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