Isolation and Structure Determination of New Antibacterial Peptide Curacomycin Based on Genome Mining
| Autor: | Hisayuki Komaki, Shinya Kodani, Issara Kaweewan, Hikaru Hemmi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Tryptophan halogenase biology Organic Chemistry Nonribosomal peptide synthetase Streptomyces noursei Nuclear magnetic resonance spectroscopy biology.organism_classification Streptomyces curacoi Cyclic peptide Amino acid cyclic peptide 03 medical and health sciences 030104 developmental biology Biochemistry chemistry Valine genome mining Leucine Isoleucine Gene |
| Zdroj: | SC30201804060026 NARO成果DBd |
| ISSN: | 2193-5807 |
| DOI: | 10.1002/ajoc.201700433 |
| Popis: | A new antibacterial cyclic peptide, named curacomycin (1), and its analogue dechlorocuracomycin (2) were isolated from Streptomyces curacoi (NBRC 12761T) and Streptomyces noursei (NBRC 15452T), respectively, by using genome mining. The chemical structures of these two peptides were determined by using a combination of MS (ESI) and NMR spectroscopy. The structure of compound 1 was determined to be a cyclic peptide that consisted of six amino acids, including: valine (Val), leucine (Leu), isoleucine (Ile), ornithine (Orn), β-hydroxyasparagine (OHAsn), and 5-chlorotryptophan (ClTrp). The NMR data of compound 2 were very similar to that of compound 1, which indicated that the structure of compound 2 was a dechlorinated analogue of compound 1. A comparison of the antimicrobial activities of these two peptides indicated that the presence of chlorine in compound 1 was critical for its antimicrobial activity. The proposed biosynthetic gene clusters for compounds 1 and 2 were found in the genome data of S. curacoi and S. noursei, respectively. The functions of the biosynthetic genes were considered by comparison of the two gene clusters. |
| Databáze: | OpenAIRE |
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