Criteria of slope and endogenous level for selection of matrix in the quantitation of endogenous compounds by LC-MS/MS

Autor:	Allena Ji Ji, Guangxin Xu, Lin Tao, Hongqin Jenny Jiao, Rachel Elisabeth Palmer
Rok vydání:	2022
Předmět:	Medical Laboratory Technology Tandem Mass Spectrometry Clinical Biochemistry Reproducibility of Results General Medicine General Pharmacology Toxicology and Pharmaceutics Biomarkers Chromatography Liquid Analytical Chemistry
Zdroj:	Bioanalysis. 14:807-816
ISSN:	1757-6199 1757-6180
Popis:	Selection of the appropriate matrix for standard curve is critical for an accurate and sensitive biomarker method. Slope of a standard curve is the key factor for parallelism assessment between tested matrix and authentic matrix for LC-MS/MS assays. Here the authors have established slope criteria using a generic equation and endogenous level criteria for achieving assay sensitivity. The slope difference criterion is from -13.0 to +17.6% for LC-MS assays with ± 15% bias criteria. When the ratio of endogenous concentration in the tested matrix to the lower limit of quantitation is
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b87a274df57c1c894459206f363019b https://doi.org/10.4155/bio-2022-0066 Zobrazit plný text záznamu