Feasibility and scalability of spring parameters in distraction enterogenesis in a murine model
| Autor: | Shant Shekherdimian, James C.Y. Dunn, Matthias Stelzner, Genia Dubrovsky, Andrew Scott, Joshua D. Rouch, Nhan Huynh |
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| Rok vydání: | 2017 |
| Předmět: |
Short Bowel Syndrome
medicine.medical_specialty Tissue Expansion Biology Mice 03 medical and health sciences Intestinal lengthening 0302 clinical medicine 030225 pediatrics Distraction Alloys medicine Animals Transplantation Tissue Expansion Devices Short bowel syndrome medicine.disease Surgery Mice Inbred C57BL Treatment Outcome Spring (device) Murine model 030220 oncology & carcinogenesis Feasibility Studies Bowel lengthening Biomedical engineering |
| Zdroj: | Journal of Surgical Research. 215:219-224 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2017.04.009 |
| Popis: | Distraction enterogenesis has been investigated as a novel treatment for short bowel syndrome (SBS). With variable intestinal sizes, it is critical to determine safe, translatable spring characteristics in differently sized animal models before clinical use. Nitinol springs have been shown to lengthen intestines in rats and pigs. Here, we show spring-mediated intestinal lengthening is scalable and feasible in a murine model.A 10-mm nitinol spring was compressed to 3 mm and placed in a 5-mm intestinal segment isolated from continuity in mice. A noncompressed spring placed in a similar fashion served as a control. Spring parameters were proportionally extrapolated from previous spring parameters to accommodate the smaller size of murine intestines. After 2-3 wk, the intestinal segments were examined for size and histology.Experimental group with spring constants, k = 0.2-1.4 N/m, showed intestinal lengthening from 5.0 ± 0.6 mm to 9.5 ± 0.8 mm (P 0.0001), whereas control segments lengthened from 5.3 ± 0.5 mm to 6.4 ± 1.0 mm (P 0.02). Diameter increased similarly in both groups. Isolated segment perforation was noted when k ≥ 0.8 N/m. Histologically, lengthened segments had increased muscularis thickness and crypt depth in comparison to normal intestine.Nitinol springs with k ≤ 0.4 N/m can safely yield nearly 2-fold distraction enterogenesis in length and diameter in a scalable mouse model. Not only does this study derive the safe ranges and translatable spring characteristics in a scalable murine model for patients with short bowel syndrome, it also demonstrates the feasibility of spring-mediated intestinal lengthening in a mouse, which can be used to study underlying mechanisms in the future. |
| Databáze: | OpenAIRE |
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