Generation and characterization of highly purified canine Schwann cells from spinal nerve dorsal roots as potential new candidates for transplantation strategies
| Autor: | Annika Lehmbecker, Yimin Wang, Wolfgang Baumgärtner, Veronika M. Stein, Ingo Gerhauser, Nicole Steffensen, Regina Carlson, Andrea Tipold |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Dorsal roots Biopsy Central nervous system Biomedical Engineering Medicine (miscellaneous) Cell Count Receptors Nerve Growth Factor Flow cytometry Biomaterials 03 medical and health sciences Dogs 0302 clinical medicine Ganglia Spinal Animals Medicine Antigens 610 Medicine & health Spinal cord injury 630 Agriculture medicine.diagnostic_test business.industry Regeneration (biology) medicine.disease Transplantation Intervertebral disk 030104 developmental biology medicine.anatomical_structure Spinal nerve Schwann Cells Spinal Nerve Roots business 030217 neurology & neurosurgery |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 12:e422-e437 |
| ISSN: | 1932-6254 |
| Popis: | Schwann cells (SCs) are promising candidates for transplantation-strategies in the central nervous system by promoting axonal regeneration. The dog represents a translational model for human spinal cord injury (SCI) for studies with new repair strategies after intervertebral disk herniation (IVDH). To overcome the necessity for an additional surgical procedure, for the first time a protocol for isolation, and purification of canine SCs from spinal-nerve-biopsies during standard hemilaminectomy in IVDH affected paraplegic dogs for potential transplantation was developed. Purity was assessed by flow cytometry. Results were compared to biopsies from dogs without SCI. Within 26 ± 4 days 90.2 ± 8.8 % p75 neurotrophin-receptor (p75NTR) positive cells were achieved in IVDH dogs. The total cell count in acute/subacute and chronic IVDH (acute/subacute: 6.82 ± 6.36 x 106; chronic: 2.29 ± 2.00 x 106) differed significantly (p = 0.0120) at the potential time point of transplantation. No differences in culture-period and purity were detected between dogs with IVDH and without. Despite the small sample size and the altered environment the isolation of SCs was successful. Negative influences on isolation and purification due to potential pathological changes at the biopsy site of IVDH diseased dogs were ruled out by comparison of SC pellets from diseased and control dogs. Finally, the functionality of SCs from dogs with IVDH was outlined in co-culture experiments with canine dorsal-root–ganglion-neurons. In conclusion, nerve-root-biopsies provide a sufficient number of highly purified and functional SCs within a useful time period for novel therapeutic strategies in dogs with SCI. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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