Hepatoprotective and antioxidant activities of Hepacare®, a herbal formulation against carbon tetrachloride-induced liver injury
| Autor: | A. A. Akinyede, Ismail O. Ishola, S. A. Omilabu, A. K. Robert |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Antioxidant DPPH medicine.medical_treatment Aspartate transaminase Pharmacology Nitric Oxide Antioxidants Superoxide dismutase Rats Sprague-Dawley chemistry.chemical_compound Inhibitory Concentration 50 Malondialdehyde Drug Discovery medicine Animals Carbon Tetrachloride Liver injury ABTS biology Dose-Response Relationship Drug Chemistry Plant Extracts General Medicine Free Radical Scavengers medicine.disease Rats Biochemistry Alanine transaminase Carbon tetrachloride biology.protein Chemical and Drug Induced Liver Injury Silymarin |
| Zdroj: | Drug research. 65(1) |
| ISSN: | 2194-9387 |
| Popis: | BACKGROUND Hepacare(®) is a herbal formulation used to treat patients with sickle-cell anaemia complicated with jaundice, also recommended as a protective agent against liver damage due to chronic ingestion of alcohol. METHODS In vitro antioxidant properties of Hepacare(®) was determined using 1, 1- diphenyl-2-picryl-hydrazyl (DPPH), total antioxidant capacity, reducing power ability, and nitric oxide assays. Hepatoprotective effect of Hepacare(®) (50-400 mg/kg/day for 7 days, p.o.) was investigated in male Sprague Dawley rats against carbon tetrachloride (CCl(4) /olive oil, 1:1, 0.7 ml/kg, i.p.)-induced liver damage. At the end of the study, blood samples and liver tissue were assayed for biochemical and antioxidants parameters. RESULTS Hepacare produced concentration dependent inhibition of DPPH and nitric oxide activity with IC(50) of 48.50 and 55.00 µg/ml, respectively, it suppressed the absorbance of ABTS(.+) with total antioxidant capacity of 423.47±8.37 mg QUE/g. CCl(4) administration induced significant (P |
| Databáze: | OpenAIRE |
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