Metabolic profiling of fatty acids in Tripterygium wilfordii multiglucoside- and triptolide-induced liver-injured rats
| Autor: | Linjing Wu, Xilan Tang, Cong Hu, Yinhua Xiong, Hongwei Li, Xiaojie Liu, Siyu Deng |
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| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
Pharmacology fatty acids General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Metabolomics tripterygium wilfordii multiglucoside medicine Biology (General) 030304 developmental biology chemistry.chemical_classification Liver injury 0303 health sciences General Immunology and Microbiology biology General Neuroscience Fatty acid Lipid metabolism Triptolide biology.organism_classification medicine.disease metabolic profile chemistry triptolide 030220 oncology & carcinogenesis Rheumatoid arthritis Tripterygium wilfordii General Agricultural and Biological Sciences Nephrotic syndrome liver injury |
| Zdroj: | Open Life Sciences, Vol 16, Iss 1, Pp 184-197 (2021) |
| ISSN: | 2391-5412 |
| Popis: | Tripterygium wilfordii multiglucoside (TWM) is a fat-soluble extract from a Chinese herb T. wilfordii, that’s used in treating rheumatoid arthritis, nephrotic syndrome and other skin diseases. Triptolide (TP) is a major active component in TWM. However, clinical applications of TWM are limited by its various toxicities especially hepatotoxicity. In recent studies, it has been reported that drug-induced liver injury (DILI) could induce the disorder of lipid metabolism in animals. Hence, this study focuses on the metabolic profile of fatty acids in TWM- and TP-induced liver-injured rats. In serum and liver tissue, 16 free and 16 esterified fatty acids were measured by gas chromatography coupled with mass spectrometry. Metabolic profile of serum fatty acids in rats with liver injury was identified by multivariate statistical analysis. The fatty acid levels in the serum of TWM- and TP-treated rats significantly decreased, whereas those in the liver tissue of TWM- and TP-treated rats obviously increased when compared with the vehicle-treated rats. Four free fatty acids were identified as candidate biomarkers of TWM- and TP-induced liver injury. Therefore, the targeted metabolomic method may be used as a complementary approach for DILI diagnosis in clinic. |
| Databáze: | OpenAIRE |
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