Mining of hemicellulose and lignin degrading genes from differentially enriched methane producing microbial community
| Autor: | Prabhakar D. Pandit, Madhuri Gulhane, Hemant J. Purohit, Anshuman A. Khardenavis |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
animal structures Environmental Engineering Caldicellulosiruptor Microbial Consortia Bioengineering 010501 environmental sciences 01 natural sciences Lignin chemistry.chemical_compound Bioreactors Biogas Polysaccharides 010608 biotechnology Hemicellulose Food science Waste Management and Disposal 0105 earth and related environmental sciences biology Bacteria Renewable Energy Sustainability and the Environment business.industry Ruminococcus Hydrolysis food and beverages Reproducibility of Results Oryza General Medicine Biodiversity biology.organism_classification Sphingomonas Biotechnology Anaerobic digestion Food waste chemistry Food Biofuels Xylanase Metagenome business Methane |
| Zdroj: | Bioresource technology. 216 |
| ISSN: | 1873-2976 |
| Popis: | Study creates a scenario for enrichment and selection of ligno-hemicellulose degrading genotypes with anaerobic bioreactor as a model using rice straw, vegetable waste and food waste as substrates. Relative discrimination analysis showed that the hydrolytic pathways and associated microbial communities for ligno-hemicellulose degradation were dominatingly colonized with rice straw as substrate. The dominating bacteria were Caldicellulosiruptor, Fervidobacterium, Cytophaga, Ruminococcus, Thermotoga associated with hemicellulose degradation and Burkholderia, Pandorea, Sphingomonas, Spirochaeta, Pseudomonas for lignocellulose hydrolysis. This was further supported by the abundance of anaerobic aromatic compound degrading genes along with genes for xylanase and xylosidase in rice straw enriched community. The metagenome analysis data was validated by evaluation of the biochemical methane potential for these substrates. Food waste being most amenable substrate yielded 1410mL of biogas/gVS added whereas, biogas yield of 1160mL/gVS and 1080mL/gVS was observed in presence of vegetable waste and rice straw respectively. |
| Databáze: | OpenAIRE |
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