Immunological Responses in Patients Who Have Spontaneously Eradicated Hepatitis C Virus Infection
| Autor: | Lihua Qu, Ann L. Silverman, Ian M. Zitron, Marius Laurinaitis, Stuart C. Gordon |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Time Factors Adolescent Hepatitis C virus Immunoblotting Immunology Hepacivirus Viral Nonstructural Proteins Biology Lymphocyte Activation medicine.disease_cause Peripheral blood mononuclear cell Serology law.invention Immunoenzyme Techniques Viral Proteins Immune system Risk Factors law Virology medicine Humans Child Substance Abuse Intravenous medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Interferon-alpha RNA Hepatitis C Antibodies Hepatitis C Chronic Hepatitis C Recombinant Proteins In vitro Liver Immunoassay Recombinant DNA RNA Viral Molecular Medicine Female |
| Zdroj: | Viral Immunology. 13:521-531 |
| ISSN: | 1557-8976 0882-8245 |
| DOI: | 10.1089/vim.2000.13.521 |
| Popis: | We examined the proliferative responses of peripheral blood mononuclear cells obtained from 60 untreated patients who were seropositive by enzyme immunoassay, but negative for hepatitis C virus (HCV) RNA by reverse transcriptase-polymerase chain reaction (RT-PCR). We used second- and third-generation recombinant immunoblot assay (RIBA) for further serological characterization. In vitro HCV-specific proliferative responses of mononuclear cells were compared to those of both untreated chronic HCV patients and patients who showed sustained virological response to interferon-alpha monotherapy, in order to assess the relative contribution of the immune response to the eradication of HCV. We found that frequency of responses to nonstructural proteins showed statistically significant differences, which were attributable to vigorous, polyspecific responses by cells from the RIBA-positive patients. In this group, core-specific proliferation was significantly associated with intravenous drug use as route of acquisition. Both other patient groups and the RIBA-indeterminate patients showed indistinguishable frequencies of proliferative responses. No association was detected between residual humoral responses, as determined from the RIBA results, and elapsed time since infection. The frequency of antibodies to NS5 differs between spontaneous cure and chronically infected patients. Cell-mediated and humoral immunity appear to be maintained in this population of patients. |
| Databáze: | OpenAIRE |
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