Comparative phosphoproteomic analysis of developing maize seeds suggests a pivotal role for enolase in promoting starch synthesis
Autor: | Yuwei Zhou, Hanwei Cao, Xiuyan Zhang, Ying Chang, Dongtao Ren, Cui Li |
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Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Sucrose Proteome Starch Mutant Plant Science Biology 01 natural sciences Zea mays 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Genetics Hexose Glycolysis Plant Proteins chemistry.chemical_classification food and beverages General Medicine Phosphoproteins Enzyme assay 030104 developmental biology Enzyme chemistry Biochemistry Phosphopyruvate Hydratase Seeds biology.protein Sorbitol Agronomy and Crop Science 010606 plant biology & botany |
Zdroj: | Plant science : an international journal of experimental plant biology. 289 |
ISSN: | 1873-2259 |
Popis: | Maize (Zea mays) seeds are the major source of starch all over the world and the excellent model for researching starch synthesis. Seed starch content is a typical quantitative phenotype and many reports revealed that the glycolytic enzymes are involved in regulating starch synthesis, however the regulatory mechanism is still unclear. Here, we present a comparative phosphoproteomic study of three maize inbred lines with different seed starch content. It reveals that abundances of 62 proteins and 63 phosphoproteins were regulated during maize seed development. Dynamics of 17 enzymes related to glycolysis and starch synthesis were used to construct a phosphorylation regulatory network of starch synthesis. It shows that starch synthesis and glycolysis in maize seeds utilize the same hexose phosphates pool coming from sorbitol and sucrose as carbon source, and phosphorylation of ZmENO1 are suggested to contribute to increase starch content, because it is positively related to seed starch content in different developmental stages and different lines, and the phosphor-mimic mutant (ZmENO1S43D) damaged its enzyme activity which is vital in glycolysis. Our results provide a new sight into regulatory process of seed starch synthesis and can be used in maize breeding for high starch content. |
Databáze: | OpenAIRE |
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