Microsatellite instability and protein expression of the DNA mismatch repair gene,hMLH1, of lung cancer in chromate-exposed workers

Autor: Masaru Tsuyuguchi, Atsushi Ochiai, Yasumasa Monden, Toshiaki Sano, Masato Hashimoto, Kazuya Kondo, Toshiyuki Hirose, Yuji Takahashi, Hidewaki Nakagawa
Rok vydání: 2005
Předmět:
Adult
Male
congenital
hereditary
and neonatal diseases and abnormalities
Cancer Research
Lung Neoplasms
DNA Repair
Base Pair Mismatch
Down-Regulation
Biology
medicine.disease_cause
Genomic Instability
Occupational Exposure
Chromates
medicine
Humans
Promoter Regions
Genetic
Lung cancer
Molecular Biology
Gene
Adaptor Proteins
Signal Transducing
Aged
Neoplasm Staging
Lung
Nuclear Proteins
nutritional and metabolic diseases
Microsatellite instability
Methylation
DNA Methylation
Middle Aged
respiratory system
medicine.disease
Molecular biology
digestive system diseases
Neoplasm Proteins
Gene Expression Regulation
Neoplastic
Occupational Diseases
MutL Proteins
medicine.anatomical_structure
Cancer research
Immunohistochemistry
DNA mismatch repair
Carrier Proteins
MutL Protein Homolog 1
Carcinogenesis
human activities
Microsatellite Repeats
Zdroj: Molecular Carcinogenesis. 42:150-158
ISSN: 1098-2744
0899-1987
DOI: 10.1002/mc.20073
Popis: Our previous studies of lung cancer in chromate-exposed workers (chromate lung cancer) have revealed that the frequency of replication error (RER) in chromate lung cancer is very high. We examined whether the RER phenotype of chromate lung cancer is due to an abnormality of DNA mismatch repair protein. We investigated the expression of a DNA mismatch repair gene, hMLH1, and hMSH2 proteins using immunohistochemistry and microsatellite instability (MSI) in 35 chromate lung cancers and 26 nonchromate lung cancers. Lung cancer without MSI or with MSI at one locus was defined as “RER(−),” lung cancer with MSI at two loci was defined as “RER(+),” and lung cancer with MSI at three or more loci was defined as “RER(++).” The repression rate of hMLH1 and hMSH2 proteins in chromate lung cancer was significantly more than that of nonchromate lung cancer (hMLH1: 56% vs. 20%, P = 0.006, hMSH2: 74% vs. 23%, P
Databáze: OpenAIRE
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