Primary structure and strand specificity of BstF5I-1 DNA methyltransferase which recognizes 5′-GGATG-3′
| Autor: | Abdurashitov Ma, Sergei Kh. Degtyarev, Shevchenko Av, N. A. Netesova |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
DNA
Bacterial Site-Specific DNA-Methyltransferase (Adenine-Specific) Molecular Sequence Data Biology DNA methyltransferase Homology (biology) Substrate Specificity law.invention Geobacillus stearothermophilus Recognition sequence law Isoschizomer Genetics Nucleotide Amino Acid Sequence Cloning Molecular Gene chemistry.chemical_classification Base Sequence Protein primary structure General Medicine DNA Methylation Molecular biology chemistry Recombinant DNA |
| Zdroj: | Gene. 187:217-219 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/s0378-1119(96)00752-4 |
| Popis: | The gene for Bst F5I-1 DNA-methyltransferase (MTase) from Bacillus stearothermophilus F5 (a Fok I isoschizomer, recognizing 5′-GGATG-3′) was cloned and its nucleotide (nt) sequence was determined. Analysis of deduced amino acid (aa) sequence shows that M· Bst F5I-1 belongs to D 21 class of MTases and has a little homology with M· Fok I. M· Bst F5I-1 modifies only the upper strand of recognition sequence (5′-GGATG-3′). |
| Databáze: | OpenAIRE |
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