A TBP–TAF complex required for transcription of human snRNA genes by RNA polymerases II and III
| Autor: | Ryuji Kobayashi, C L Sadowski, Nouria Hernandez, R. W. Henry |
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| Rok vydání: | 1995 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data genetic processes information science RNA polymerase II macromolecular substances Cross Reactions RNA Polymerase I RNA Small Nuclear Humans Amino Acid Sequence RNA polymerase II holoenzyme Genetics Multidisciplinary Base Sequence biology General transcription factor TATA-Box Binding Protein DNA Bacteriophage lambda TATA Box Recombinant Proteins Cell biology DNA-Binding Proteins health occupations biology.protein Transcription factor II F RNA Polymerase II Transcription factor II E Transcription factor II D Transcription factor II A HeLa Cells Transcription Factors |
| Zdroj: | Nature. 374:653-656 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/374653a0 |
| Popis: | The TATA-box-binding protein TBP exists in the cell complexed with different sets of TBP-associated factors (TAFs). In general, each of these TBP-TAF complexes is dedicated to transcription by a single RNA polymerase. Thus, SL1, TFIID and TFIIIB are required for transcription by polymerases I, II and III, respectively. Here we characterize a fourth TBP-TAF complex called SNAPc. Unlike the other TBP-TAF complexes, SNAPc is implicated in transcription by two types of polymerases; it is required for transcription of both the RNA polymerase II and III small-nuclear RNA genes and binds specifically to the proximal sequence element PSE, a non-TATA-box basal promoter element common to these two types of genes. In addition to TBP, SNAPc is composed of at least three TAFs, SNAP43, SNAP45 and SNAP50. The predicted amino-acid sequence of SNAP43 reveals that it corresponds to a new protein. |
| Databáze: | OpenAIRE |
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