Effect of Different Regimens of Co-Transplantation of Hemopoietic and Mesenchymal Hemopoietic Stem Cells on the Rates of Hemopoietic Recovery in Laboratory Mice Treated with Cyclophosphamide in Sublethal Doses
| Autor: | V L Ivanov, L. P. Zhavoronkov, Selivanova Ei, L. N. Pavlova, A. G. Konoplyannikov, V. V. Pavlov, O F Chibisova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cyclophosphamide medicine.drug_class Biology Monoclonal antibody General Biochemistry Genetics and Molecular Biology Andrology 03 medical and health sciences Mice 0302 clinical medicine medicine Animals Platelet CD117 Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine Flow Cytometry Hematopoiesis Mice Inbred C57BL Haematopoiesis 030104 developmental biology medicine.anatomical_structure biology.protein Mice Inbred CBA Female Bone marrow Stem cell 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Bulletin of experimental biology and medicine. 166(4) |
| ISSN: | 1573-8221 |
| Popis: | We studied the effect of mesenchymal stem cells and hemopoietic stem cells co-transplanted in different regimens (sequence and intervals between the injections within 0-48 h) on the rate of the bone marrow hemopoiesis recovery in 468 inbred mice with cytostatic aplasia caused by a single injection of cyclophosphamide in a dose of 500 mg/kg. The efficacy of stem cells was evaluated by animal survival and general physical condition, body weight dynamics, peripheral blood counts (leucocytes, platelets, and reticulocytes), total cellularity and the presence of hemopoietic stem cells in the bone marrow on day 9 after cyclophosphamide injection. The relative content of hemopoietic stem cells in the total myelokaryocyte pool was assessed by flow cytofluorometry using specific monoclonal antibodies to CD117 and CD184. The stimulating effect of co-transplantation of mesenchymal stem cells and hemopoietic stem cells on hemopoiesis was demonstrated by the indices of total cellularity and the relative content of CD117+ hemopoietic stem cells. The schemes with injection of mesenchymal stem cells 24-48 h prior to injection of hemopoietic stem cells were most effective. |
| Databáze: | OpenAIRE |
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