Comprehensive analysis of the differential expression profile of microRNAs in rats with spinal cord injury treated by electroacupuncture

Autor: Cao Cao, Li Hejian, Hongchun Li, Fu Kaiwen, Jing Zhang, Zhou Zhidong, Jun Luo, Deng Fumou
Rok vydání: 2019
Předmět:
0301 basic medicine
Male
Cancer Research
Electroacupuncture
medicine.medical_treatment
Apoptosis
Wounds
Penetrating
Pharmacology
Biochemistry
Rats
Sprague-Dawley
0302 clinical medicine
reverse transcription-qPCR
Databases
Genetic
Gene Regulatory Networks
microRNA profile
Spinal cord injury
Wnt signaling pathway
High-Throughput Nucleotide Sequencing
Articles
Hindlimb
medicine.anatomical_structure
Oncology
Spinal Cord
030220 oncology & carcinogenesis
Molecular Medicine
Biology
03 medical and health sciences
Downregulation and upregulation
microRNA
electroacupuncture
Genetics
medicine
Animals
RNA
Messenger
Molecular Biology
Spinal Cord Injuries
Inflammation
Oncogene
Computational Biology
Recovery of Function
medicine.disease
Spinal cord
Molecular medicine
spinal cord injury
Rats
Disease Models
Animal
MicroRNAs
030104 developmental biology
next-generation sequencing
Transcriptome
Zdroj: Molecular Medicine Reports
ISSN: 1791-3004
Popis: Abnormal microRNA (miRNA) expression has been implicated in spinal cord injury (SCI), but the underlying mechanisms are poorly understood. To observe the effect of electroacupuncture (EA) on miRNA expression profiles in SCI rats and investigate the potential mechanisms involved in this process, Sprague‑Dawley rats were divided into sham, SCI and SCI+EA groups (n=6 each). Basso, Beattie and Bresnahan (BBB) scoring and hematoxylin‑eosin staining of cortical tissues were used to evaluate spinal cord recovery with EA treatment 21 days post‑surgery across the three groups. To investigate miRNA expression profiles, 6 Sprague‑Dawley rats were randomly divided into SCI and SCI+EA groups (n=3 in each group) and examined using next‑generation sequencing. Integrated miRNA‑mRNA‑pathway network analysis was performed to elucidate the interaction network of the candidate miRNAs, their target genes and the involved pathways. Behavioral scores suggested that hindlimb motor functions improved with EA treatments. Apoptotic indices were lower in the SCI+EA group compared with the SCI group. It was also observed that 168 miRNAs were differentially expressed between the SCI and SCI+EA groups, with 29 upregulated and 139 downregulated miRNAs in the SCI+EA group. Changes in miRNA expression are involved in SCI physiopathology, including inflammation and apoptosis. Reverse transcription‑quantitative PCR measurement of the five candidate miRNAs, namely rno‑miR‑219a‑5p, rno‑miR‑486, rno‑miR‑136‑5p, rno‑miR‑128‑3p, and rno‑miR‑7b, was consistent with RNA sequencing data. Integrated miRNA‑mRNA‑pathway analysis suggested that the MAPK, Wnt and NF‑κB signaling pathways were involved in EA‑mediated recovery from SCI. The present study evaluated the miRNA expression profiles involved in EA‑treated SCI rats and demonstrated the potential mechanism and functional role of miRNAs in SCI in rats.
Databáze: OpenAIRE
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