Effects of Shugan-Huayu powder, a traditional Chinese medicine, on hepatic fibrosis in rat model
Autor: | Lung-Yuan Wu, Wen-Jane Lee, Kwong-Chung Tung, Nai-Nu Lin, Yung-Tsung Chiu, Hsiao-Ping Hsu, Ching-Chang Cheng, Yen-Feng Lee |
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Rok vydání: | 2011 |
Předmět: |
Male
medicine.medical_specialty Physiology CCL4 Matrix Metalloproteinase Inhibitors Chronic liver disease Liver Cirrhosis Experimental Salvia miltiorrhiza Rats Sprague-Dawley Transforming Growth Factor beta1 Western blot Physiology (medical) Internal medicine medicine Animals Zymography Medicine Chinese Traditional Carbon Tetrachloride Sho-saiko-to medicine.diagnostic_test business.industry hemic and immune systems medicine.disease Actins Rats Disease Models Animal Endocrinology Liver Hepatic stellate cell Matrix Metalloproteinase 2 Powders Hepatic fibrosis business |
Zdroj: | The Chinese journal of physiology. 53(4) |
ISSN: | 0304-4920 |
Popis: | Shugan-Huayu powder (SHP) has been administered to outpatients with chronic liver disease without clear anti-fibrosis mechanism. To investigate the anti-fibrotic effects of SHP on liver fibrosis in a rat model and in hepatic stellate cells (HSCs) in vitro, rats were gavaged with CCl4 at 1.0 g/kg body weight twice a week for 8 weeks to induce liver fibrosis and the rats were randomly assigned to one of the three groups: -CCl4 alone, low-dose SHP and high-dose SHP. SHP was given by gavages 5 times a week for 8 weeks. Serum, livers and HSCs were assayed for serology, pathology, western blot, zymography and quantitative RT-PCR. Hepatic function improved as decreased serum aspartate aminotransferase and alanine aminotransferase, and collagen deposition and active HSCs were significantly reduced in CCl4-induced liver by SHP treatment. The expression of matrix metalloproteinase-2 (MMP-2) and transforming growth factor-beta1 (TGF-beta1) mRNA in fibrotic liver showed significant downregulation after SHP treatment. In vitro, inhibition of alpha-smooth muscle actin (alpha-SMA) expression and MMP-2 secretion of active HSCs were also noticed by SHP treatment. SHP has an antifibrotic effect on CCl4-induced liver fibrosis in rats. Anti-fibrotic mechanisms were probably inhibiting activation of HSCs and decreased expression of MMP-2 and TGF-beta1. |
Databáze: | OpenAIRE |
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