Ligands, Receptors, and Transcription Factors that Mediate Inter-Cellular and Intra-Cellular Communication during Ovarian Follicle Development
Autor: | Lonnie D. Shea, Teresa K. Woodruff, Linda J. Broadbelt, Beatriz Penalver Bernabe |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Biology Transcriptome 03 medical and health sciences Paracrine signalling Mice 0302 clinical medicine Ovarian Follicle Paracrine Communication medicine Animals Ovarian follicle Autocrine signalling Transcription factor 030219 obstetrics & reproductive medicine Obstetrics and Gynecology Gene Expression Regulation Developmental Oocyte Cell biology Autocrine Communication 030104 developmental biology medicine.anatomical_structure Theca Female Original Article Signal transduction Signal Transduction Transcription Factors |
Zdroj: | Reprod Sci |
ISSN: | 1933-7205 |
Popis: | Reliably producing a competent oocyte entails a deeper comprehension of ovarian follicle maturation, a very complex process that includes meiotic maturation of the female gamete, the oocyte, together with the mitotic divisions of the hormone-producing somatic cells. In this report, we investigate murine ovarian folliculogenesis in vivo using publicly available time-series microarrays from primordial to antral stage follicles. Manually curated protein interaction networks were employed to identify autocrine and paracrine signaling between the oocyte and the somatic cells (granulosa and theca cells) at multiple stages of follicle development. We established plausible protein-binding interactions between expressed genes that encode secreted factors and expressed genes that encode cellular receptors. Some computationally identified signaling interactions are well established, such as the paracrine signaling from the oocyte to the somatic cells through the oocyte-secreted growth factor Gdf9, while others are novel connections in term of ovarian folliculogenesis, such as the possible paracrine connection from somatic-secreted factor Ntn3 to the oocyte receptor Neo1. Additionally, we identified several of the likely transcription factors that might control the dynamic transcriptome during ovarian follicle development, noting that the YAP/TAZ signaling pathway is very active in vivo. This novel dynamic model of signaling and regulation can be employed to generate testable hypotheses regarding follicle development that could be validated experimentally, guiding the improvement of culture media to enhance in vitro ovarian follicle maturation and possibly novel therapeutic targets for reproductive diseases. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s43032-019-00075-8) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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