B-Helper Neutrophils in Regional Lymph Nodes Correlate with Improved Prognosis in Patients with Head and Neck Cancer

Autor: Elena Siakaeva, Agnes Bankfalvi, Georg Korschunow, Benjamin A. Kansy, Maksim Domnich, Stephan Lang, Ilona Spyra, Charlotte Wallner, Jadwiga Jablonska, Magdalena Korek, Irem Ozel, E A Pylaeva, Anthony Squire, Moritz Goetz
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Cancer Research
head-and-neck cancer
Medizin
Article
Medizinische Fakultät » Universitätsklinikum Essen » Institut für Experimentelle Immunologie und Bildgebung
Flow cytometry
NBH
03 medical and health sciences
0302 clinical medicine
neutrophils
lymph nodes
neutrophils -- B cells -- B helper neutrophils -- NBH -- head-and-neck cancer -- lymph nodes -- regional lymph nodes -- head-and-neck squamous cell carcinoma
Medicine
ddc:610
regional lymph nodes
B cell
RC254-282
Medizinische Fakultät » Universitätsklinikum Essen » Klinik für Hals-Nasen-Ohrenheilkunde
B cells
medicine.diagnostic_test
B helper neutrophils
business.industry
Head and neck cancer
Cancer
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Medizinische Fakultät » Universitätsklinikum Essen » Institut für Pathologie
medicine.disease
Acquired immune system
Head and neck squamous-cell carcinoma
head-and-neck squamous cell carcinoma
030104 developmental biology
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Cancer research
Immunohistochemistry
Lymph
business
Zdroj: Cancers
Volume 13
Issue 12
Cancers, Vol 13, Iss 3092, p 3092 (2021)
ISSN: 2072-6694
DOI: 10.3390/cancers13123092
Popis: Simple Summary Neutrophils exhibit multiple functions during cancer progression and are believed to regulate adaptive immune responses to cancer. In addition to their interactions with T cells in this context, these cells are also believed to interact with B cells. Neutrophils have been found in the marginal zone of the spleen, where they exhibit helper cell characteristics, supporting B cell proliferation and activation. Here, we investigate the effect of neutrophils on B cells in the regional lymph nodes (RLN) of head-and-neck cancer (HNC) patients. We have identified that, in RLNs, neutrophils express a helper cell phenotype that was associated with the increased activation and proliferation of B cells. Importantly, the high abundance of neutrophils in the B cell follicles of regional lymph nodes is associated with significantly improved HNC patient survival. Abstract The role of neutrophils during cancer formation and elimination is diverse. Here, for the first time, we investigate neutrophil helper cells (NBH), their influence on B cell activity in the regional lymph nodes (RLN) of head-and-neck cancer patients and the effect of this neutrophil/B cell interaction on patient prognosis. Circulating and RLN neutrophils of patients with stage I–IV head-and-neck squamous cell carcinoma were investigated with flow cytometry and qPCR. In addition, neutrophil/B cell co-localization in RLNs was evaluated using immunohistochemistry. B cell proliferation was assessed and correlated with the distance to neutrophils. Patient survival was evaluated. Neutrophils with the helper cell phenotype were identified in the RLN of HNC patients. B cells in close proximity to such NBH showed significantly higher proliferation rates, together with elevated activation-induced cytidine deaminase (AID) expression. Notably, patient survival was significantly higher in individuals with high NBH frequencies in the B follicles of RLNs. Neutrophils in RLN can support T cell-independent activation of the adaptive immune system through B cell stimulation, capturing helper cell phenotype character. The presence of such helper neutrophils in the RLNs of HNC patients positively correlates with patient prognosis.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje