Detection of Viral Antigens in Formalin-fixed Specimens by Enzyme Treatment
| Autor: | Takeshi Kurata, Shoichiro Sato, Yuzo Aoyama, Ryo Hondo, Joseph B. McCormick, Akira Oda |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Adult
Male Adolescent viruses Fluorescent Antibody Technique Immunofluorescence Measles General Biochemistry Genetics and Molecular Biology Subacute sclerosing panencephalitis Serology Fixatives Mice Chickenpox Lassa Fever History and Philosophy of Science Antigen Formaldehyde Animals Humans Medicine Child Encephalitis Japanese Antigens Viral Aged medicine.diagnostic_test business.industry General Neuroscience Progressive multifocal leukoencephalopathy Leukoencephalopathy Progressive Multifocal Acute hemorrhagic conjunctivitis virus diseases Herpes Simplex Middle Aged Conjunctivitis medicine.disease Virology Japanese B Encephalitis Cytomegalovirus Infections Female Subacute Sclerosing Panencephalitis business |
| Zdroj: | Annals of the New York Academy of Sciences. 420:192-207 |
| ISSN: | 1749-6632 0077-8923 |
| DOI: | 10.1111/j.1749-6632.1983.tb22204.x |
| Popis: | Enzyme treatment (protease or trypsin) was applied to formalin-fixed paraffin-embedded materials and virus-infected cultured cells to detect viral antigens by immunofluorescence. The viral antigens were demonstrated in several organs of autopsy or biopsy cases of which diagnoses had been established by immunofluorescence or virus isolation using frozen materials, or suspected on the basis of serology and/or histopathological findings. These included herpes simplex, varicella-zoster, cytomegalo, subacute sclerosing panencephalitis, progressive multifocal leukoencephalopathy, Japanese B encephalitis, measles, acute hemorrhagic conjunctivitis, Lassa and Korean hemorrhagic fever. Antigen could be recovered also in virus-infected cells (herpes simplex, measles, Lassa, Ebola, Marburg, Rift Valley, Congo and Korean Hemorrhagic fever) by enzyme treatment after periods of formalin fixation of four weeks and storage of three months. In herpes simplex virus-infected mouse brain, antigen was detected after fixation for three months in formalin. |
| Databáze: | OpenAIRE |
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