An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
| Autor: | Christian Fernandes, Gerson Antônio Pianetti, Pedro Henrique Reis da Silva, Paula Cristina Rezende Enéas |
|---|---|
| Jazyk: | portugalština |
| Rok vydání: | 2017 |
| Předmět: |
Chromatography
Chemistry Content determination High performance liquid chromatography/validation Analytical chemistry Linearity lcsh:RS1-441 Capsules 02 engineering and technology Química High-performance liquid chromatography Linearity assessment Clinical Practice lcsh:Pharmacy and materia medica 020210 optoelectronics & photonics Prednisone Prednisona 0202 electrical engineering electronic engineering information engineering medicine General Pharmacology Toxicology and Pharmaceutics Hplc method Cápsulas (Farmácia) medicine.drug |
| Zdroj: | Repositório Institucional da UFMG Universidade Federal de Minas Gerais (UFMG) instacron:UFMG Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 2 (2017); e16064 Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 2 (2017); e16064 Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 2 (2017); e16064 Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo (USP) instacron:USP Brazilian Journal of Pharmaceutical Sciences, Vol 53, Iss 2 (2017) Brazilian Journal of Pharmaceutical Sciences, Volume: 53, Issue: 2, Article number: e16064, Published: 22 JUN 2017 |
| ISSN: | 2175-9790 1984-8250 |
| Popis: | Prednisone is an anti-inflammatory steroid drug widely used in clinical practice. However, no high-performance liquid chromatographic (HPLC) method has been described in the literature for the determination of prednisone in capsules until now. Thus, an HPLC method was developed using a C18 (250x4.0, 5 µm) column, with methanol:water (70:30) as mobile phase at a flow rate of 1 mL/min and detection at 240 nm. The developed method was validated following current Brazilian legislation. Additionally, linearity was assessed by evaluating the assumptions of normality, homoscedasticity, and independency of residuals, and the fit to the linear model. The method showed linearity (r2>0.99) over the range of 14.0-26.0 µg/mL, selectivity, precision (RSD |
| Databáze: | OpenAIRE |
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