| Popis: |
A method for the measurement of the total amount of androstenedione in urine, both free and conjugated. has been devised. After mild acid hydrolysis and a paper Chromatographic purification stage, the amount of androstenedione has been estimated by radioimmunoassay. The mean excretion of androstenedione for 12 male subjects was 198 nmol/24 h. and for 11 female subjects, 77.4 nmol/24 h. A good correlation was found between the total urinary excretion of androstenedione and the mean plasma androstenedione concentration during the same period, for a group of postmenopausal subjects, indicating that the measurement of total urinary androstenedione is a useful reflection of plasma androstenedione values and thus androstenedione secretion through a 24 h period. |
