Biosynthesis of Terpenoids: Efficient Multistep Biotransformation Procedures Affording Isotope-Labeled 2C-Methyl-d -erythritol 4-Phosphate Using Recombinant 2C-Methyl-d -erythritol 4-Phosphate Synthase
| Autor: | Stefan Hecht, Christoph A. Schuhr, Tanja Radykewicz, Wolfgang Eisenreich, Adelbert Bacher, Gerald Richter, Felix Rohdich, Juraithip Wungsintaweekul, Klaus Kis |
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| Rok vydání: | 2001 |
| Předmět: |
Tritium
medicine.disease_cause law.invention chemistry.chemical_compound Bacterial Proteins Biotransformation Biosynthesis Multienzyme Complexes law Escherichia coli medicine Carbon Radioisotopes Gene Aldose-Ketose Isomerases ATP synthase biology Terpenes Chemistry Organic Chemistry food and beverages 2C-methyl-D-erythritol 4-phosphate synthase Recombinant Proteins Terpenoid Erythritol Biochemistry Isotope Labeling biology.protein Recombinant DNA Sugar Phosphates Oxidoreductases |
| Zdroj: | The Journal of Organic Chemistry. 66:7770-7775 |
| ISSN: | 1520-6904 0022-3263 |
| DOI: | 10.1021/jo015890v |
| Popis: | This paper describes the recombinant expression of the ispC gene of Escherichia coli specifying 2C-methyl-D-erythritol 4-phosphate synthase in a modified form that can be purified efficiently by metal-chelating chromatography. The enzyme was used for the preparation of isotope-labeled 2C-methyl-D-erythritol 4-phosphate employing isotope-labeled glucose and pyruvate as starting materials. The simple one-pot methods described afford numerous isotopomers of 2C-methyl-D-erythritol 4-phosphate carrying (3)H, (13)C, or (14)C from commercially available precursors. The overall yield based on the respective isotope-labeled starting material is approximately 50%. |
| Databáze: | OpenAIRE |
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