Infection of Dendritic Cells (DCs), Not DC-SIGN-Mediated Internalization of Human Immunodeficiency Virus, Is Required for Long-Term Transfer of Virus to T Cells

Autor: Cecile Schiffer, Isabelle Staropoli, Valérie Pezo, Françoise Porrot, Ali Amara, Pierre-Yves Lozach, Bruno Canque, Jean-Louis Virelizier, Laura Burleigh, Fernando Arenzana-Seisdedos
Přispěvatelé: Immunologie Virale, Institut Pasteur [Paris], Unité mixte de recherche virologie moléculaire et structurale, Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Hopital Saint-Louis [AP-HP] (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Biologie Cellulaire de l'Immunite Antitumorale, Institut National de la Santé et de la Recherche Médicale (INSERM), Virus et Immunité, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), This work was supported by grants from SIDACTION and ANRS (Agence Nationale de Recherches sur le SIDA). L.B. and P.-Y.L. were funded by fellowships from SIDACTION and the Pediatric Dengue Vaccine Initiative, respectively., Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
CD4-Positive T-Lymphocytes
HIV Infections
MESH: Amino Acid Sequence
MESH: HIV-1
MESH: Animals
Internalization
media_common
MESH: Receptors
Cell Surface
0303 health sciences
biology
MESH: Dendritic Cells
MESH: CD4-Positive T-Lymphocytes
MESH: HIV Infections
3. Good health
Cell biology
Virus-Cell Interactions
DC-SIGN
Lentivirus
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
MESH: Cell Adhesion Molecules
media_common.quotation_subject
Immunology
Molecular Sequence Data
Receptors
Cell Surface
Endocytosis
Microbiology
Virus
Cell Line
03 medical and health sciences
Virology
Animals
Humans
Lectins
C-Type
Amino Acid Sequence
030304 developmental biology
MESH: Molecular Sequence Data
MESH: Humans
030306 microbiology
T lymphocyte
Dendritic cell
Dendritic Cells
biology.organism_classification
MESH: Cell Line
Cell culture
Insect Science
biology.protein
HIV-1
Cell Adhesion Molecules
MESH: Lectins
C-Type
Zdroj: Journal of Virology
Journal of Virology, American Society for Microbiology, 2006, 80 (6), pp.2949-2957. ⟨10.1128/JVI.80.6.2949-2957.2006⟩
Journal of Virology, 2006, 80 (6), pp.2949-2957. ⟨10.1128/JVI.80.6.2949-2957.2006⟩
ISSN: 0022-538X
1098-5514
DOI: 10.1128/JVI.80.6.2949-2957.2006⟩
Popis: The C-type lectin DC-SIGN expressed on immature dendritic cells (DCs) captures human immunodeficiency virus (HIV) particles and enhances the infection of CD4 + T cells. This process, known as trans -enhancement of T-cell infection, has been related to HIV endocytosis. It has been proposed that DC-SIGN targets HIV to a nondegradative compartment within DCs and DC-SIGN-expressing cells, allowing incoming virus to persist for several days before infecting target cells. In this study, we provide several lines of evidence suggesting that intracellular storage of intact virions does not contribute to HIV transmission. We show that endocytosis-defective DC-SIGN molecules enhance T-cell infection as efficiently as their wild-type counterparts, indicating that DC-SIGN-mediated HIV internalization is dispensable for trans -enhancement. Furthermore, using immature DCs that are genetically resistant to infection, we demonstrate that several days after viral uptake, HIV transfer from DCs to T cells requires viral fusion and occurs exclusively through DC infection and transmission of newly synthesized viral particles. Importantly, our results suggest that DC-SIGN participates in this process by cooperating with the HIV entry receptors to facilitate cis -infection of immature DCs and subsequent viral transfer to T cells. We suggest that such a mechanism, rather than intracellular storage of incoming virus, accounts for the long-term transfer of HIV to CD4 + T cells and may contribute to the spread of infection by DCs.
Databáze: OpenAIRE
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