Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells
| Autor: | Jacques Grober, Vanessa van Harmelen, Peter Arner, Dominique Langin, Signy Reynisdottir, Cecilia Holm, Valérie Large |
|---|---|
| Přispěvatelé: | Institut Louis Bugnard, Large, Valerie |
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Male
Hormone-sensitive lipase White adipose tissue Biochemistry MESH: Isoproterenol Adenylyl cyclase chemistry.chemical_compound 0302 clinical medicine Endocrinology Adipocyte Adipocytes MESH: Obesity MESH: Genetic Variation MESH: Cell Size 0303 health sciences Forskolin MESH: Sterol Esterase biology MESH: Kinetics Immunochemistry MESH: Molecular Weight food and beverages Female catecholamines Adult medicine.medical_specialty Lipolysis 030209 endocrinology & metabolism QD415-436 In Vitro Techniques 03 medical and health sciences white adipose tissue Internal medicine medicine Humans MESH: Lipolysis Obesity RNA Messenger Lipase MESH: Adipocytes 030304 developmental biology Cell Size MESH: RNA Messenger MESH: In Vitro Techniques MESH: Humans Activator (genetics) MESH: Immunochemistry Isoproterenol Genetic Variation MESH: Adult Cell Biology Sterol Esterase β-adrenoceptors MESH: Male Molecular Weight [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition Kinetics chemistry lipid mobilization biology.protein MESH: Female [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition |
| Zdroj: | Journal of Lipid Research Journal of Lipid Research, American Society for Biochemistry and Molecular Biology, 1998, 39 (8), pp.1688-95 Journal of Lipid Research, Vol 39, Iss 8, Pp 1688-1695 (1998) Karolinska Institutet Europe PubMed Central |
| ISSN: | 0022-2275 |
| Popis: | Hormone-sensitive lipase (HSL) catalyzes the rate-limiting step in adipocyte lipolysis. The activity of HSL is thought to be primarily regulated by reversible phosphorylation. However, the regulation of HSL activity by pretranslational mechanisms has been poorly studied. The present studies were undertaken to explore the relationship between the levels of HSL protein and mRNA expressions and the lipolytic capacity. The study was performed in human abdominal subcutaneous adipocytes with identical sizes but having either a high (HL) or low (LL) lipolytic capacity (n = 16). Basal and maximal lipolysis induced by catecholamines, an adenylyl cyclase activator forskolin, and a cyclic AMP analogue dibutyryl cAMP were 50% lower in LL- in comparison with HL-fat cells (P < 0.05 or better). No differences in drug sensitivity were found. HSL activity and quantity were about 50% lower in LL- compared with HL-fat cells (P < 0.05). Moreover, the mRNA ratio between HSL and γ-actin was 35% lower in LL-compared with HL-fat cells (P < 0.05). There was a strong linear correlation between the protein and enzymatic HSL measurements (r2 = 0.91). In addition, the maximum lipolytic capacity was significantly correlated with HSL activity (r 2 = 0.75) and HSL protein amount (r 2 = 0.64). It is concluded that hormone-sensitive lipase (HSL) expression, measured either as total HSL protein by Western blot analysis or as total amount of activatable HSL enzyme, is a major determinant of the maximum lipolytic capacity of human fat cells. In addition, HSL protein expression is at least, in part, determined by HSL mRNA expression.—Large, V., P. Arner, S. Reynisdottir, J. Grober, V. Van Harmelen, C. Holm, and D. Langin. Hormone-sensitive lipase expression and activity in relation to lipolysis in human fat cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|