A β-sheet structure interacting peptide for intracellular protein delivery into human pluripotent stem cells and their derivatives
| Autor: | Shu Wang, Seong Loong Lo, Shixiong Lua, Jianxing Song |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Pluripotent Stem Cells Biocompatibility Cell Membrane Biophysics Beta sheet Proteins Peptide Cell Biology Biology Biochemistry Embryonic stem cell Neural stem cell Protein Structure Secondary Cell biology Protein Transport chemistry Cell Line Tumor Amphiphile Humans Stem cell Induced pluripotent stem cell Peptides Molecular Biology Nuclear Magnetic Resonance Biomolecular |
| Zdroj: | Biochemical and biophysical research communications. 421(3) |
| ISSN: | 1090-2104 |
| Popis: | The advance in stem cell research relies largely on the efficiency and biocompatibility of technologies used to manipulate stem cells. In our previous study, we had designed an amphipathic peptide RV24 that can deliver proteins into cancer cell lines efficiently without significant side effects. Encouraged by this observation, we moved forward to test whether RV24 could be used to deliver proteins into human embryonic stem cells and human induced pluripotent stem cells. RV24 successfully mediated protein delivery into these pluripotent stem cells, as well as their derivatives including neural stem cells and dendritic cells. Based on NMR studies and particle surface charge measurements, we proposed that hydrophobic domain of RV24 interacts with β-sheet structures of the proteins, followed by formation of “peptide cage” to facilitate delivery across cellular membrane. These findings suggest the feasibility of using amphipathic peptide to deliver functional proteins intracellularly for stem cell research. |
| Databáze: | OpenAIRE |
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