Insulin Degradation: IX. On the Presence of Glutathione-insulin Transhydrogenase in Human Leukocytes
| Autor: | Partab T. Varandani, Michael L. Chandler |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Immunodiffusion
Erythrocytes Endocrinology Diabetes and Metabolism Biology Cell membrane chemistry.chemical_compound Leukocytes Internal Medicine medicine Humans Insulin Antigens Insulin degradation Glucose tolerance test Human blood Human liver medicine.diagnostic_test Cell Membrane Glutathione Glucose Tolerance Test Glutathione-insulin transhydrogenase Kinetics Glutathione Reductase medicine.anatomical_structure Biochemistry chemistry Ethylmaleimide biology.protein Antibody |
| Zdroj: | Diabetes. 23:232-239 |
| ISSN: | 1939-327X 0012-1797 |
| DOI: | 10.2337/diab.23.3.232 |
| Popis: | The occurrence of insulin-degrading activity in the components of human blood has been studied. Leukocytes have been found to contain such activity. On the basis of activation by reduced glutathione, complete inhibition by N-ethyl-maleimide, kinetic studies, and reaction with antibody to purified human liver glutathione-insulin transhydrogenase (GIT), it is concluded that the insulin-degrading activity present in leukocytes is, in fact, GIT. The data indicate that erythrocytes contain a very small amount of insulin-degrading activity; this activity is probably associated with the cell membrane. Preliminary studies in normal human volunteers indicate that there are apparent differences between individuals in the leukocyte GIT content. No evidence was found for any consistent changes in leukocyte GIT content during the course of a glucose tolerance test. |
| Databáze: | OpenAIRE |
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