Differentiation of Prothymocytes Induced by Trypsin
| Autor: | H. Hugh Fudenberg, E. H. Eylar |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Cytotoxicity
Immunologic T-Lymphocytes Immunology Mice Nude Spleen Thymus Extracts Mice Nude mouse Cell surface receptor medicine Animals Trypsin Incubation Kunitz STI protease inhibitor biology Cell Differentiation biology.organism_classification Molecular biology In vitro Thymocyte medicine.anatomical_structure Biochemistry Antigens Surface Thy-1 Antigens Female medicine.drug |
| Zdroj: | Immunological Communications. 13:305-312 |
| ISSN: | 0090-0877 |
| DOI: | 10.3109/08820138409048665 |
| Popis: | Treatment in vitro of nude mouse spleen prothymocytes with relatively low concentrations (1-50 micrograms/ml) of trypsin induce the Thy 1- to Thy 1+ conversion. The effect of trypsin was inhibited by prior heating or by addition of soybean trypsin inhibitor. The maximal effect achieved by trypsin treatment, approximately 25% conversion of spleen cells from layer B of an albumin gradient into theta-positive cells, was equivalent to that obtained with thymic hormone preparation (TP-1) at 10 ng/ml. Maximal conversion required 120 min incubation with TP-1 or trypsin. We conclude that both trypsin and TP-1 act by perturbation of a membrane receptor which can send a signal initiating the differentiation process. |
| Databáze: | OpenAIRE |
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