Superswelling microneedle arrays for dermal interstitial fluid (prote) omics
| Autor: | Alfonso Nieto-Argüello, Elise Laszlo, Davide Brambilla, Gregory De Crescenzo, Xavier Banquy |
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| Přispěvatelé: | Université de Montréal. Faculté de pharmacie |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
0303 health sciences Materials science Superabsorbant hydrogel 02 engineering and technology 021001 nanoscience & nanotechnology Condensed Matter Physics Omics Electronic Optical and Magnetic Materials Biomaterials 03 medical and health sciences Interstitial fluid sampling Interstitial fluid Electrochemistry 0210 nano-technology 030304 developmental biology Biomedical engineering Microneedles |
| DOI: | 10.1002/adfm.202106061 |
| Popis: | The noninvasive sampling of dermal interstitial fluid (ISF) for the monitoring of clinical biomarkers is a greatly appealing area of research. The identification of molecular biomarkers in biological fluids has been accelerated with -omics analyses but remains limited in ISF because of its time-consuming and complex extraction process. Here, the generation of microneedle (MN) patches made of superabsorbent acrylate-based hydrogels for the rapid sampling of dermal ISF is described to explore its proteome. In depth, iterative optimization allows the identification of novel acrylate-based compositions with the required chemical, mechanical, and biocompatibility properties allowing proteomic analysis of the extracted ISF for the first time after sampling with swelling MNs. The generated MN arrays show no cytotoxic effect, successfully cross the stratum corneum, and can collect up to 6 µL of dermal ISF in 10 min in vivo. Proteomics lead to the detection of 176 clinically relevant biomarkers in the collected samples validating the use of ISF as a relevant bodily fluid for disease monitoring and diagnostic. Importantly, it is discovered that extraction fingerprint is strongly dependent on the MNs chemistry, and thus specific biomarkers could be selectively extracted by tuning the composition of the patch, making the system versatile and specific. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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