Mechanical Stress Inhibits Early Stages of Endogenous Cell Migration: A Pilot Study in an Ex Vivo Osteochondral Model
| Autor: | Sibylle Grad, Mauro Alini, Gerjo J.V.M. van Osch, M.L. Vainieri, Avner Yayon |
|---|---|
| Přispěvatelé: | Orthopedics and Sports Medicine, Otorhinolaryngology and Head and Neck Surgery |
| Rok vydání: | 2020 |
| Předmět: |
MMP3
Stromal cell Polymers and Plastics 0206 medical engineering Cell 02 engineering and technology Matrix metalloproteinase Article lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry Downregulation and upregulation medicine cartilage endogenous cell recruitment 030304 developmental biology mechanical loading 0303 health sciences Chemistry Regeneration (biology) biomaterial osteochondral Cell migration General Chemistry 020601 biomedical engineering Cell biology medicine.anatomical_structure hydrogel Ex vivo |
| Zdroj: | Polymers, Vol 12, Iss 1754, p 1754 (2020) Polymers Volume 12 Issue 8 Polymers, 12 (8) Polymers, 12(8) Polymers, 12(8):1754. Multidisciplinary Digital Publishing Institute (MDPI) |
| ISSN: | 2073-4360 |
| DOI: | 10.3390/polym12081754 |
| Popis: | Cell migration has a central role in osteochondral defect repair initiation and biomaterial-mediated regeneration. New advancements to reestablish tissue function include biomaterials and factors promoting cell recruitment, differentiation and tissue integration, but little is known about responses to mechanical stimuli. In the present pilot study, we tested the influence of extrinsic forces in combination with biomaterials releasing chemoattractant signals on cell migration. We used an ex vivo mechanically stimulated osteochondral defect explant filled with fibrin/hyaluronan hydrogel, in presence or absence of platelet-derived growth factor-BB or stromal cell-derived factor 1, to assess endogenous cell recruitment into the wound site. Periodic mechanical stress at early time point negatively influenced cell infiltration compared to unloaded samples, and the implementation of chemokines to increase cell migration was not efficient to overcome this negative effect. The gene expression at 15 days of culture indicated a marked downregulation of matrix metalloproteinase (MMP)13 and MMP3, a decrease of β1 integrin and increased mRNA levels of actin in osteochondral samples exposed to complex load. This work using an ex vivo osteochondral mechanically stimulated advanced platform demonstrated that recurrent mechanical stress at early time points impeded cell migration into the hydrogel, providing a unique opportunity to improve our understanding on management of joint injury. Polymers, 12 (8) ISSN:2073-4360 |
| Databáze: | OpenAIRE |
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