Phospholipid Scramblase 1 Modulates FcR-Mediated Phagocytosis in Differentiated Macrophages
| Autor: | Serge Benichou, Jérôme Bouchet, Cécile Hérate, Nancy J. Grant, Sabrina Marion, Stéphane Gasman, Florence Niedergang, Ghania Ramdani |
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| Přispěvatelé: | Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Cellulaires et Intégratives (INCI), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), univOAK, Archive ouverte |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Phospholipid scramblase Phagocytosis Cellular differentiation Down-Regulation lcsh:Medicine Bone Marrow Cells Phosphatidylserines Receptors Fc [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Biology Monocytes Cell membrane 03 medical and health sciences chemistry.chemical_compound Mice [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] medicine [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Macrophage Animals Humans Phospholipid Transfer Proteins RNA Small Interfering lcsh:Science Cells Cultured Phagosome Mice Knockout Multidisciplinary Monocyte Macrophages Cell Membrane lcsh:R Cell Differentiation Phosphatidylserine Molecular biology Cell biology 030104 developmental biology medicine.anatomical_structure chemistry Microscopy Fluorescence [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] RNA Interference lcsh:Q Research Article HeLa Cells |
| Zdroj: | PLoS ONE PLoS ONE, 2016, 11 (1), pp.e0145617. ⟨10.1371/journal.pone.0145617⟩ PLoS ONE, Vol 11, Iss 1, p e0145617 (2016) PLoS ONE, Public Library of Science, 2016, 11 (1), pp.e0145617. ⟨10.1371/journal.pone.0145617⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0145617⟩ |
| Popis: | Phospholipid Scramblase 1 (PLSCR1) was initially characterized as a type II transmembrane protein involved in bilayer movements of phospholipids across the plasma membrane leading to the cell surface exposure of phosphatidylserine, but other cellular functions have been ascribed to this protein in signaling processes and in the nucleus. In the present study, expression and functions of PLSCR1 were explored in specialized phagocytic cells of the monocyte/macrophage lineage. The expression of PLSCR1 was found to be markedly increased in monocyte-derived macrophages compared to undifferentiated primary monocytes. Surprisingly, this 3-fold increase in PLSCR1 expression correlated with an apparent modification in the membrane topology of the protein at the cell surface of differentiated macrophages. While depletion of PLSCR1 in the monocytic THP-1 cell-line with specific shRNA did not inhibit the constitutive cell surface exposure of phosphatidylserine observed in differentiated macrophages, a net increase in the FcR-mediated phagocytic activity was measured in PLSCR1-depleted THP-1 cells and in bone marrow-derived macrophages from PLSCR1 knock-out mice. Reciprocally, phagocytosis was down-regulated in cells overexpressing PLSCR1. Since endogenous PLSCR1 was recruited both in phagocytic cups and in phagosomes, our results reveal a specific role for induced PLSCR1 expression in the modulation of the phagocytic process in differentiated macrophages. |
| Databáze: | OpenAIRE |
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