Generation of human blastocyst-like structures from pluripotent stem cells
| Autor: | Pu-Yao Zhang, E. Zhang, Samhan M. Alsolami, Mo Li, Yongliang Wang, Chenrui An, Yong Fan, Wei Chen, Wendi Pei, Hai-Ying Zhu, Zhenglai Ma, Xiangjin Kang, Linfeng Zhan, Rong Li, Jie Qiao, Tao Tan, Zheying Min, Yingying Zhang, Ke Zhong, Yang Yu |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Pluripotency
Embryonic stem cells QH573-671 Embryo Cell Biology Biology Biochemistry Embryonic stem cell Article Cell biology Transcriptome medicine.anatomical_structure Epiblast Embryonic Structure embryonic structures Genetics medicine Human embryogenesis Blastocyst Cytology Induced pluripotent stem cell Molecular Biology reproductive and urinary physiology |
| Zdroj: | Cell Discovery Cell Discovery, Vol 7, Iss 1, Pp 1-14 (2021) |
| ISSN: | 2056-5968 |
| Popis: | Human blastocysts are comprised of the first three cell lineages of the embryo: trophectoderm, epiblast and primitive endoderm, all of which are essential for early development and organ formation. However, due to ethical concerns and restricted access to human blastocysts, a comprehensive understanding of early human embryogenesis is still lacking. To bridge this knowledge gap, a reliable model system that recapitulates early stages of human embryogenesis is needed. Here we developed a three-dimensional (3D), two-step induction protocol for generating blastocyst-like structures (EPS-blastoids) from human extended pluripotent stem (EPS) cells. Morphological and single-cell transcriptomic analyses revealed that EPS-blastoids contain key cell lineages and are transcriptionally similar to human blastocysts. Furthermore, EPS-blastoids are similar with human embryos that were cultured for 8 or 10 days in vitro, in terms of embryonic structures, cell lineages and transcriptomic profiles. In conclusion, we developed a scalable system to mimic human blastocyst development, which can potentially facilitate the study of early implantation failure that induced by developmental defects at early stage. |
| Databáze: | OpenAIRE |
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