Observation of intracellular potassium and sodium in the heart by NMR: a major fraction of potassium is 'invisible'
| Autor: | Herbert Hoefeler, Eric T. Fossel |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Intracellular Fluid
Magnetic Resonance Spectroscopy Chemistry Sodium Potassium 23na nmr Myocardium Analytical chemistry chemistry.chemical_element Fraction (chemistry) Rats Inbred Strains In Vitro Techniques Rats Perfusion Microelectrode Reagent Animals Radiology Nuclear Medicine and imaging Intracellular potassium Intracellular Nuclear chemistry |
| Zdroj: | Magnetic resonance in medicine. 3(4) |
| ISSN: | 0740-3194 |
| Popis: | Using 39K and 23Na NMR in conjunetion with extracellularly localized shift reagents, we have determined the intracellular concentrations of NMR visible sodium and potassium in isolated, perfused rat hearts. We find this concentration to be 9.9 mM/kg cell water for sodium and 31 mM/kg cell water for potassium. Values of activity determined by ionsensitive microelectrodes are in good agreement with our sodium value but do not agree with our potassium value. Our results mean that a major pool of intracelluar potassium is, on average, signifieantly immobilized and that the mobile NMR visible faction (31mM/kg) is not in exchange with the NMR invisible pool (114mM/kg). The immobilized fraction is characterized by T2 values which are too short to be observed by our conventional spectrometer. This fraction is, therefore, said to be “invisible” under our experimental conditions. © 1986 Academic Press, Inc. |
| Databáze: | OpenAIRE |
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