Transcriptomic profiles ofDrosophila melanogasterthird instar larval midgut and responses to oxidative stress
| Autor: | Omprakash Mittapalli, H. M. Li, Grzegorz Buczkowski, Jun Xie, Rick Westerman, Brandon J. Schemerhorn, Barry R. Pittendrigh, Larry L. Murdock, Jing Wu |
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| Rok vydání: | 2008 |
| Předmět: |
Proteases
digestive system Cytochrome P-450 Enzyme System Genetics Melanogaster Animals Molecular Biology Gene Glutathione Transferase Oligonucleotide Array Sequence Analysis Expressed Sequence Tags Genome Base Sequence biology Gene Expression Profiling Serine Endopeptidases fungi Proteolytic enzymes Cytochrome P450 Midgut Hydrogen Peroxide biology.organism_classification Gastrointestinal Tract Oxidative Stress Drosophila melanogaster Glutathione S-transferase Gene Expression Regulation Larva Insect Science biology.protein Carboxylic Ester Hydrolases |
| Zdroj: | Insect Molecular Biology. 17:325-339 |
| ISSN: | 1365-2583 0962-1075 |
| DOI: | 10.1111/j.1365-2583.2008.00808.x |
| Popis: | Oligoarray analysis was used to determine the number and nature of genes expressed in third instar Drosophila melanogaster larval midguts. The majority of transcripts were associated with protein synthesis and metabolism. Serine proteases were the main proteolytic enzymes detected. Some 40% of the cytochrome P450 genes and 74% of the glutathione S transferases (GSTs) in the genome of D. melanogaster were observed to be expressed in the midgut by oligoarray analysis. We also identified potential transcription factor binding motifs (TFBMs) of P450s, GSTs and carboxylesterases. Many of the midgut-expressed GST genes contained candidate TFBMs homologous to TFBMs in mammals that have been associated with responses to oxidative stress. We also investigated the response of GSTs in the midgut to dietary H 2 O 2 , which showed a dosagebased differential response. |
| Databáze: | OpenAIRE |
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